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Molecular detection of bioluminescent dinoflagellates in surface waters of the Patagonian Shelf during early austral summer 2008

Molecular detection of bioluminescent dinoflagellates in surface waters of the Patagonian Shelf during early austral summer 2008
Molecular detection of bioluminescent dinoflagellates in surface waters of the Patagonian Shelf during early austral summer 2008
We investigated the distribution of bioluminescent dinoflagellates in the Patagonian Shelf region using “universal” PCR primers for the dinoflagellate luciferase gene. Luciferase gene sequences and single cell PCR tests, in conjunction with taxonomic identification by microscopy, allowed us to identify and quantify bioluminescent dinoflagellates. We compared these data to coincidental discrete optical measurements of stimulable bioluminescence intensity. Molecular detection of the luciferase gene showed that bioluminescent dinoflagellates were widespread across the majority of the Patagonian Shelf region. Their presence was comparatively underestimated by optical bioluminescence measurements, whose magnitude was affected by interspecific differences in bioluminescence intensity and by the presence of other bioluminescent organisms. Molecular and microscopy data showed that the complex hydrography of the area played an important role in determining the distribution and composition of dinoflagellate populations. Dinoflagellates were absent south of the Falkland Islands where the cold, nutrient-rich, and well-mixed waters of the Falklands Current favoured diatoms instead. Diverse populations of dinoflagellates were present in the warmer, more stratified waters of the Patagonian Shelf and Falklands Current as it warmed northwards. Here, the dinoflagellate population composition could be related to distinct water masses. Our results provide new insight into the prevalence of bioluminescent dinoflagellates in Patagonian Shelf waters and demonstrate that a molecular approach to the detection of bioluminescent dinoflagellates in natural waters is a promising tool for ecological studies of these organisms.
1932-6203
e98849
Valiadi, M.
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Painter, S.C.
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Allen, J.T.
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Balch, W.M.
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Iglesias-Rodriguez, M.D.
f93f3be3-83b0-46c1-a5f8-e42ad1e30674
Valiadi, M.
e245e9c5-3e97-4896-b910-c4178c5fb3c9
Painter, S.C.
29e32f35-4ee8-4654-b305-4dbe5a312295
Allen, J.T.
b251a62b-f443-4591-b695-9aa8c4d73741
Balch, W.M.
9f519f3f-949a-481b-83ed-6d856745c9c9
Iglesias-Rodriguez, M.D.
f93f3be3-83b0-46c1-a5f8-e42ad1e30674

Valiadi, M., Painter, S.C., Allen, J.T., Balch, W.M. and Iglesias-Rodriguez, M.D. (2014) Molecular detection of bioluminescent dinoflagellates in surface waters of the Patagonian Shelf during early austral summer 2008. PLoS ONE, 9 (6), e98849.

Record type: Article

Abstract

We investigated the distribution of bioluminescent dinoflagellates in the Patagonian Shelf region using “universal” PCR primers for the dinoflagellate luciferase gene. Luciferase gene sequences and single cell PCR tests, in conjunction with taxonomic identification by microscopy, allowed us to identify and quantify bioluminescent dinoflagellates. We compared these data to coincidental discrete optical measurements of stimulable bioluminescence intensity. Molecular detection of the luciferase gene showed that bioluminescent dinoflagellates were widespread across the majority of the Patagonian Shelf region. Their presence was comparatively underestimated by optical bioluminescence measurements, whose magnitude was affected by interspecific differences in bioluminescence intensity and by the presence of other bioluminescent organisms. Molecular and microscopy data showed that the complex hydrography of the area played an important role in determining the distribution and composition of dinoflagellate populations. Dinoflagellates were absent south of the Falkland Islands where the cold, nutrient-rich, and well-mixed waters of the Falklands Current favoured diatoms instead. Diverse populations of dinoflagellates were present in the warmer, more stratified waters of the Patagonian Shelf and Falklands Current as it warmed northwards. Here, the dinoflagellate population composition could be related to distinct water masses. Our results provide new insight into the prevalence of bioluminescent dinoflagellates in Patagonian Shelf waters and demonstrate that a molecular approach to the detection of bioluminescent dinoflagellates in natural waters is a promising tool for ecological studies of these organisms.

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Published date: June 2014
Organisations: Ocean and Earth Science, Marine Biogeochemistry

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Local EPrints ID: 359745
URI: http://eprints.soton.ac.uk/id/eprint/359745
ISSN: 1932-6203
PURE UUID: 31b3728d-b790-46e4-aae7-268d4a1d35fc

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Date deposited: 11 Nov 2013 13:15
Last modified: 11 Nov 2019 20:53

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