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Augmenting endogenous wnt signaling improves skin wound healing

Augmenting endogenous wnt signaling improves skin wound healing
Augmenting endogenous wnt signaling improves skin wound healing
Wnt signaling is required for both the development and homeostasis of the skin, yet its contribution to skin wound repair remains controversial. By employing Axin2(LacZ/+) reporter mice we evaluated the spatial and temporal distribution patterns of Wnt responsive cells, and found that the pattern of Wnt responsiveness varies with the hair cycle, and correlates with wound healing potential. Using Axin2(LacZ/LacZ) mice and an ear wound model, we demonstrate that amplified Wnt signaling leads to improved healing. Utilizing a biochemical approach that mimics the amplified Wnt response of Axin2(LacZ/LacZ) mice, we show that topical application of liposomal Wnt3a to a non-healing wound enhances endogenous Wnt signaling, and results in better skin wound healing. Given the importance of Wnt signaling in the maintenance and repair of skin, liposomal Wnt3a may have widespread application in clinical practice.
1932-6203
e76883
Whyte, Jemima L.
0b1d56c2-ab6c-436e-a23d-a5d88795f486
Smith, Andrew A.
c84c2111-48b5-4c8e-8b30-bfc6db080d12
Liu, Bo
b4376e27-629b-4700-b104-3548c71f35f8
Manzano, Wilfred R.
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Evans, Nick D.
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Dhamdhere, Girija R.
1d97dee5-9e9d-4a52-a438-9d7fa7e818e9
Fang, Mark Y.
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Chang, Howard Y.
49571e5f-f433-40fa-ac4e-5c0c8048f184
Oro, Anthony E.
343aa059-27c4-4938-bb70-266cad155918
Helms, Jill A.
39e95260-c5ae-4e98-902a-445c44b8e6bd
Whyte, Jemima L.
0b1d56c2-ab6c-436e-a23d-a5d88795f486
Smith, Andrew A.
c84c2111-48b5-4c8e-8b30-bfc6db080d12
Liu, Bo
b4376e27-629b-4700-b104-3548c71f35f8
Manzano, Wilfred R.
10f401a9-040c-438b-bbd2-b697d761c4a6
Evans, Nick D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Dhamdhere, Girija R.
1d97dee5-9e9d-4a52-a438-9d7fa7e818e9
Fang, Mark Y.
141749bb-0c3b-4d15-a992-06d0773d0c4e
Chang, Howard Y.
49571e5f-f433-40fa-ac4e-5c0c8048f184
Oro, Anthony E.
343aa059-27c4-4938-bb70-266cad155918
Helms, Jill A.
39e95260-c5ae-4e98-902a-445c44b8e6bd

Whyte, Jemima L., Smith, Andrew A., Liu, Bo, Manzano, Wilfred R., Evans, Nick D., Dhamdhere, Girija R., Fang, Mark Y., Chang, Howard Y., Oro, Anthony E. and Helms, Jill A. (2013) Augmenting endogenous wnt signaling improves skin wound healing. PLoS ONE, 8 (10), e76883. (doi:10.1371/journal.pone.0076883). (PMID:10817753)

Record type: Article

Abstract

Wnt signaling is required for both the development and homeostasis of the skin, yet its contribution to skin wound repair remains controversial. By employing Axin2(LacZ/+) reporter mice we evaluated the spatial and temporal distribution patterns of Wnt responsive cells, and found that the pattern of Wnt responsiveness varies with the hair cycle, and correlates with wound healing potential. Using Axin2(LacZ/LacZ) mice and an ear wound model, we demonstrate that amplified Wnt signaling leads to improved healing. Utilizing a biochemical approach that mimics the amplified Wnt response of Axin2(LacZ/LacZ) mice, we show that topical application of liposomal Wnt3a to a non-healing wound enhances endogenous Wnt signaling, and results in better skin wound healing. Given the importance of Wnt signaling in the maintenance and repair of skin, liposomal Wnt3a may have widespread application in clinical practice.

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Published date: 2013
Organisations: Bioengineering Group, Human Development & Health

Identifiers

Local EPrints ID: 359883
URI: http://eprints.soton.ac.uk/id/eprint/359883
ISSN: 1932-6203
PURE UUID: ece917db-ea68-4b71-9768-a1b1b0dfc3e2
ORCID for Nick D. Evans: ORCID iD orcid.org/0000-0002-3255-4388

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Date deposited: 15 Nov 2013 11:38
Last modified: 28 Oct 2023 02:02

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Contributors

Author: Jemima L. Whyte
Author: Andrew A. Smith
Author: Bo Liu
Author: Wilfred R. Manzano
Author: Nick D. Evans ORCID iD
Author: Girija R. Dhamdhere
Author: Mark Y. Fang
Author: Howard Y. Chang
Author: Anthony E. Oro
Author: Jill A. Helms

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