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Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts

Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts
Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts
Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.
1470-8728
217-227
Willett, Mark
dfa36c04-719a-4884-87cd-2ae13a3d2b67
Brocard, Michele
f9863062-b39f-42a0-bbd8-1184f2d90b9b
Davide, Alexandre
9e13f124-9433-4f74-91a4-6b1c8ffacb84
Morley, Simon J.
059a6269-c9e5-4165-8498-238153537ceb
Willett, Mark
dfa36c04-719a-4884-87cd-2ae13a3d2b67
Brocard, Michele
f9863062-b39f-42a0-bbd8-1184f2d90b9b
Davide, Alexandre
9e13f124-9433-4f74-91a4-6b1c8ffacb84
Morley, Simon J.
059a6269-c9e5-4165-8498-238153537ceb

Willett, Mark, Brocard, Michele, Davide, Alexandre and Morley, Simon J. (2011) Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts. Biochemical Journal, 438 (1), 217-227. (doi:10.1042/BJ20110435). (PMID:21539520)

Record type: Article

Abstract

Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.

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More information

Published date: 15 August 2011
Organisations: Centre for Biological Sciences

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Local EPrints ID: 360111
URI: http://eprints.soton.ac.uk/id/eprint/360111
ISSN: 1470-8728
PURE UUID: a143bd80-ea42-4406-93b6-c53d6277fb21

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Date deposited: 27 Nov 2013 13:57
Last modified: 16 Jul 2019 21:16

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