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Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

Triplex-mediated analysis of cytosine methylation at CpA sites in DNA
Triplex-mediated analysis of cytosine methylation at CpA sites in DNA
Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.
1359-7345
551-553
Johannsen, Marie W.
be54dc2e-f7b8-4a09-b835-c9a9adff4bdd
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Johannsen, Marie W.
be54dc2e-f7b8-4a09-b835-c9a9adff4bdd
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Johannsen, Marie W., Gerrard, Simon R., Melvin, Tracy and Brown, Tom (2014) Triplex-mediated analysis of cytosine methylation at CpA sites in DNA. Chemical Communications, 50 (5), 551-553. (doi:10.1039/C3CC45917A).

Record type: Article

Abstract

Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.

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Accepted/In Press date: 6 November 2013
e-pub ahead of print date: 7 November 2013
Published date: 18 January 2014
Organisations: Optoelectronics Research Centre, Chemistry

Identifiers

Local EPrints ID: 360139
URI: http://eprints.soton.ac.uk/id/eprint/360139
ISSN: 1359-7345
PURE UUID: 237bc4fe-7fe6-4436-9708-185f4da5f426

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Date deposited: 27 Nov 2013 13:25
Last modified: 18 Feb 2020 17:31

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Contributors

Author: Marie W. Johannsen
Author: Simon R. Gerrard
Author: Tracy Melvin
Author: Tom Brown

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