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Increased TNF expression in CD43++ murine blood monocytes

Increased TNF expression in CD43++ murine blood monocytes
Increased TNF expression in CD43++ murine blood monocytes
Monocyte heterogeneity has been studied extensively in man but only recently tools have been developed to study blood monocyte populations in the mouse. We have used the MacGreen mouse model, which expresses the green fluorescent protein under the control of the promoter of the murine M-CSF receptor (CSF1 receptor, c-fms). Since both monocytes and granulocytes show GFP expression in this model the latter cells were excluded by staining with the Ly6G granulocyte marker. GFP+ Ly6G- blood monocytes were found to account for an average of 246+/-121cells/microl in these mice. These monocytes can be subdivided into CD43+ GR-1+ cells and CD43++ GR-1(-) cells, with the latter cells accounting for 140+/-77cells/mul, i.e. about 60% of all blood monocytes. After intraperitoneal injection of lipopolysaccharide (LPS) both blood monocyte subpopulations were depleted. The same was true after intranasal infection with Streptococcus pneumoniae but here the CD43++ subpopulation was preferentially reduced to 4cells/mul. For the study of TNF expression cells were stimulated in vitro with LPS from Salmonella abortus equi in the presence of Brefeldin A followed by intracellular staining and multicolor flow cytometry. Over a dose range of 10-100ng LPS/ml, TNF protein production was significantly higher in the CD43++ monocyte subset. At 1000ng LPS/ml 90% of all CD43++ monocytes stained positive for TNF and in terms of fluorescence intensity TNF was 5-fold higher compared to the CD43+ monocytes. These data indicate that the murine CD43++ monocyte subset exhibits features of pro-inflammatory monocytes and is functionally homologeous to the human CD14+CD16+ monocytes.
0165-2478
142-147
Burke, B.
2cdd32d9-cd1f-4b70-8896-46704f009a0b
Ahmad, R.
d7aaa8e7-2817-4ad3-b3b2-c5831772a31a
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Snowden, R.
f80f1f8d-c966-4ff1-80cf-f11615a9be44
Kadioglu, A.
521a2dc6-761c-4cf4-a59d-713e04b577f6
Frankenberger, M.
b6dbd3a3-5dd4-4008-bb5b-e834476f85fa
Hume, David A.
dd2085e5-83d3-454a-9e68-9391c09133a4
Ziegler-Heitbrock, Loems
71bb9dd4-262c-46ba-9c10-1be5941ffca4
Burke, B.
2cdd32d9-cd1f-4b70-8896-46704f009a0b
Ahmad, R.
d7aaa8e7-2817-4ad3-b3b2-c5831772a31a
Staples, Karl J.
e0e9d80f-0aed-435f-bd75-0c8818491fee
Snowden, R.
f80f1f8d-c966-4ff1-80cf-f11615a9be44
Kadioglu, A.
521a2dc6-761c-4cf4-a59d-713e04b577f6
Frankenberger, M.
b6dbd3a3-5dd4-4008-bb5b-e834476f85fa
Hume, David A.
dd2085e5-83d3-454a-9e68-9391c09133a4
Ziegler-Heitbrock, Loems
71bb9dd4-262c-46ba-9c10-1be5941ffca4

Burke, B., Ahmad, R., Staples, Karl J., Snowden, R., Kadioglu, A., Frankenberger, M., Hume, David A. and Ziegler-Heitbrock, Loems (2008) Increased TNF expression in CD43++ murine blood monocytes. Immunology Letters, 118 (2), 142-147. (doi:10.1016/j.imlet.2008.03.012). (PMID:18468696)

Record type: Article

Abstract

Monocyte heterogeneity has been studied extensively in man but only recently tools have been developed to study blood monocyte populations in the mouse. We have used the MacGreen mouse model, which expresses the green fluorescent protein under the control of the promoter of the murine M-CSF receptor (CSF1 receptor, c-fms). Since both monocytes and granulocytes show GFP expression in this model the latter cells were excluded by staining with the Ly6G granulocyte marker. GFP+ Ly6G- blood monocytes were found to account for an average of 246+/-121cells/microl in these mice. These monocytes can be subdivided into CD43+ GR-1+ cells and CD43++ GR-1(-) cells, with the latter cells accounting for 140+/-77cells/mul, i.e. about 60% of all blood monocytes. After intraperitoneal injection of lipopolysaccharide (LPS) both blood monocyte subpopulations were depleted. The same was true after intranasal infection with Streptococcus pneumoniae but here the CD43++ subpopulation was preferentially reduced to 4cells/mul. For the study of TNF expression cells were stimulated in vitro with LPS from Salmonella abortus equi in the presence of Brefeldin A followed by intracellular staining and multicolor flow cytometry. Over a dose range of 10-100ng LPS/ml, TNF protein production was significantly higher in the CD43++ monocyte subset. At 1000ng LPS/ml 90% of all CD43++ monocytes stained positive for TNF and in terms of fluorescence intensity TNF was 5-fold higher compared to the CD43+ monocytes. These data indicate that the murine CD43++ monocyte subset exhibits features of pro-inflammatory monocytes and is functionally homologeous to the human CD14+CD16+ monocytes.

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Published date: 30 June 2008
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 360203
URI: https://eprints.soton.ac.uk/id/eprint/360203
ISSN: 0165-2478
PURE UUID: 898e1061-44bc-4b66-b1c8-b923e66ead7c
ORCID for Karl J. Staples: ORCID iD orcid.org/0000-0003-3844-6457

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Date deposited: 28 Nov 2013 14:45
Last modified: 06 Jun 2018 12:39

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