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Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats

Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats
Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats
BACKGROUND & AIMS: The importance of route of administration of omega-3 (n-3) polyunsaturated fatty acids (PUFA) (oral vs intravenous (iv)) is not clear. We determined the relative concentrations of fatty acids in plasma phosphatidylcholine (PC), red blood cells (RBC), white blood cells (WBC) and several tissues after short-term oral or iv administration of soybean oil (SO) or fish oil (FO).

METHODS: Wistar rats (n = 6/group) received saline, FO, or SO by gavage or saline, FO based-lipid emulsion (FLE), or SO based-lipid emulsion (SLE) iv. The oils were provided at 0.2 g/kg/day for three consecutive days. The animals were sacrificed 24 h after the last administration, blood was collected for plasma, WBC and RBC separation and tissues removed. Fatty acids were analysed by gas chromatography.

RESULTS: FO resulted in higher eicosapentaenoic acid (EPA) in plasma PC and liver than the control. FLE resulted in higher EPA, docosahexaenoic acid (DHA) and total n-3 PUFA in plasma PC, WBC and liver than both the control and SLE groups. EPA, DHA and total n-3 PUFA were higher in the heart with FLE compared with SLE. Individual and total n-3 PUFA were higher in plasma PC, WBC, liver and heart with FLE than with FO given by gavage.

CONCLUSION: Short-term iv administration of n-3 PUFA appears to be more effective at increasing EPA and DHA status in plasma, WBC, liver and heart than oral administration. This might be important for rapid treatment with n-3 PUFA.
0261-5614
993-998
Barros, K.V.
55d9f53d-840a-4322-aced-0dd224396daa
Carvalho, P.O.
3287cd24-f53d-4716-896d-5582d705511d
Cassulino, A.P.
1148c26f-cca5-4910-ad82-09a5f7d83459
Andrade, I.
424de26b-269f-4904-ac25-50fe970ec4f9
West, Annette L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Silveira, V.L.
cbcfba1a-3370-431f-8956-2dc0c1dfe8e6
Barros, K.V.
55d9f53d-840a-4322-aced-0dd224396daa
Carvalho, P.O.
3287cd24-f53d-4716-896d-5582d705511d
Cassulino, A.P.
1148c26f-cca5-4910-ad82-09a5f7d83459
Andrade, I.
424de26b-269f-4904-ac25-50fe970ec4f9
West, Annette L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Silveira, V.L.
cbcfba1a-3370-431f-8956-2dc0c1dfe8e6

Barros, K.V., Carvalho, P.O., Cassulino, A.P., Andrade, I., West, Annette L., Miles, Elizabeth A., Calder, P.C. and Silveira, V.L. (2013) Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats. Clinical Nutrition, 32 (6), 993-998. (doi:10.1016/j.clnu.2013.02.010). (PMID:23541913)

Record type: Article

Abstract

BACKGROUND & AIMS: The importance of route of administration of omega-3 (n-3) polyunsaturated fatty acids (PUFA) (oral vs intravenous (iv)) is not clear. We determined the relative concentrations of fatty acids in plasma phosphatidylcholine (PC), red blood cells (RBC), white blood cells (WBC) and several tissues after short-term oral or iv administration of soybean oil (SO) or fish oil (FO).

METHODS: Wistar rats (n = 6/group) received saline, FO, or SO by gavage or saline, FO based-lipid emulsion (FLE), or SO based-lipid emulsion (SLE) iv. The oils were provided at 0.2 g/kg/day for three consecutive days. The animals were sacrificed 24 h after the last administration, blood was collected for plasma, WBC and RBC separation and tissues removed. Fatty acids were analysed by gas chromatography.

RESULTS: FO resulted in higher eicosapentaenoic acid (EPA) in plasma PC and liver than the control. FLE resulted in higher EPA, docosahexaenoic acid (DHA) and total n-3 PUFA in plasma PC, WBC and liver than both the control and SLE groups. EPA, DHA and total n-3 PUFA were higher in the heart with FLE compared with SLE. Individual and total n-3 PUFA were higher in plasma PC, WBC, liver and heart with FLE than with FO given by gavage.

CONCLUSION: Short-term iv administration of n-3 PUFA appears to be more effective at increasing EPA and DHA status in plasma, WBC, liver and heart than oral administration. This might be important for rapid treatment with n-3 PUFA.

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Published date: December 2013
Organisations: Human Development & Health

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Local EPrints ID: 360214
URI: https://eprints.soton.ac.uk/id/eprint/360214
ISSN: 0261-5614
PURE UUID: d2b99a67-c51f-4fc2-a00b-cd75a07b9125
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655

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Date deposited: 29 Nov 2013 13:49
Last modified: 06 Jun 2018 13:13

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Contributors

Author: K.V. Barros
Author: P.O. Carvalho
Author: A.P. Cassulino
Author: I. Andrade
Author: Annette L. West
Author: Elizabeth A. Miles ORCID iD
Author: P.C. Calder
Author: V.L. Silveira

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