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Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia

Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia
Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia
Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l?1), moderate hypoxia (3·0 mg O2 l?1), normoxia (7·1 mg O2 l?1) and hyperoxia (10·6 mg O2 l?1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two-week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.
dissolved oxygen, killifish, LDH, protein, RNA:DNA, specific growth rate
0022-1112
148-164
Rees, B.B.
a20a4f2e-45de-43f0-9ffd-9641bc800fa8
Targett, T.E.
7c2f9c56-5a26-4e29-a5bb-388fc1240335
Ciotti, B.J.
034551e4-d770-4cd2-80cc-b95c677938d2
Tolman, C.A.
9eb7cce1-e606-4c8a-a90e-2a0814838604
Akkina, S.S.
9c47c2a0-e961-4485-a850-051c6eaaab8a
Gallaty, A.M.
581d2308-b54a-4593-a8d8-331618a7d319
Rees, B.B.
a20a4f2e-45de-43f0-9ffd-9641bc800fa8
Targett, T.E.
7c2f9c56-5a26-4e29-a5bb-388fc1240335
Ciotti, B.J.
034551e4-d770-4cd2-80cc-b95c677938d2
Tolman, C.A.
9eb7cce1-e606-4c8a-a90e-2a0814838604
Akkina, S.S.
9c47c2a0-e961-4485-a850-051c6eaaab8a
Gallaty, A.M.
581d2308-b54a-4593-a8d8-331618a7d319

Rees, B.B., Targett, T.E., Ciotti, B.J., Tolman, C.A., Akkina, S.S. and Gallaty, A.M. (2012) Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia. Journal of Fish Biology, 81, 148-164. (doi:10.1111/j.1095-8649.2012.03319.x). (PMID:22747810)

Record type: Article

Abstract

Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l?1), moderate hypoxia (3·0 mg O2 l?1), normoxia (7·1 mg O2 l?1) and hyperoxia (10·6 mg O2 l?1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two-week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.

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More information

Published date: July 2012
Keywords: dissolved oxygen, killifish, LDH, protein, RNA:DNA, specific growth rate
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 360281
URI: http://eprints.soton.ac.uk/id/eprint/360281
ISSN: 0022-1112
PURE UUID: 5416455e-61ea-4e68-936b-428982d69996

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Date deposited: 03 Dec 2013 09:37
Last modified: 27 Apr 2022 12:08

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Contributors

Author: B.B. Rees
Author: T.E. Targett
Author: B.J. Ciotti
Author: C.A. Tolman
Author: S.S. Akkina
Author: A.M. Gallaty

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