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Effect of vitamin A on bone resorption: evidence for direct stimulation of isolated chicken osteoclasts by retinol and retinoic acid

Effect of vitamin A on bone resorption: evidence for direct stimulation of isolated chicken osteoclasts by retinol and retinoic acid
Effect of vitamin A on bone resorption: evidence for direct stimulation of isolated chicken osteoclasts by retinol and retinoic acid
The effects of retinol (vitamin A) and retinoic acid on primary cultures of isolated chicken osteoclasts have been studied. The experiments were performed to establish the direct actions of these two agents on the organization of cytoskeletal structures, on the acid phosphatase contents, and on the bone resorption activities of these cells. The results showed that by treating the cultures with retinol or retinoic acid, from 10(-8) to 10(-5) M, there were dose-related responses of the osteoclasts. Adhesion to the substratum was stimulated by increasing the number of cells exhibiting the specialized dot-like adhesion structures, or podosomes, which represent the active part of the sealing zone. The treatments also induced rearrangement of the microtubular patterns with reversible depolymerization of microtubules. Acid phosphatase activity was significantly higher both in vitamin A-treated osteoclasts and in their media. When [3H]proline-labeled bone particles were added to the retinoid-treated osteoclasts, the release of [3H]proline was increased significantly compared to controls. These results suggest that the two vitamin A metabolites cause several modifications of the metabolic status of isolated osteoclasts that result in augmented rates of bone resorption.
0884-0431
203-210
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Teti, A.
8d6e861b-0361-4186-a6d7-c8dc515d3070
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Francis, M.J.
c207e79b-ef95-487e-895a-3d2d0476d6a5
Carano, A.
deceb531-0242-4160-be82-155c9ed5f576
Zallone, A.Z.
41630b47-07c2-4d42-9373-7026d91a1abb
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Teti, A.
8d6e861b-0361-4186-a6d7-c8dc515d3070
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Francis, M.J.
c207e79b-ef95-487e-895a-3d2d0476d6a5
Carano, A.
deceb531-0242-4160-be82-155c9ed5f576
Zallone, A.Z.
41630b47-07c2-4d42-9373-7026d91a1abb

Oreffo, R.O., Teti, A., Triffitt, J.T., Francis, M.J., Carano, A. and Zallone, A.Z. (1988) Effect of vitamin A on bone resorption: evidence for direct stimulation of isolated chicken osteoclasts by retinol and retinoic acid. Journal of Bone and Mineral Research, 3 (2), 203-210. (PMID:3063069)

Record type: Article

Abstract

The effects of retinol (vitamin A) and retinoic acid on primary cultures of isolated chicken osteoclasts have been studied. The experiments were performed to establish the direct actions of these two agents on the organization of cytoskeletal structures, on the acid phosphatase contents, and on the bone resorption activities of these cells. The results showed that by treating the cultures with retinol or retinoic acid, from 10(-8) to 10(-5) M, there were dose-related responses of the osteoclasts. Adhesion to the substratum was stimulated by increasing the number of cells exhibiting the specialized dot-like adhesion structures, or podosomes, which represent the active part of the sealing zone. The treatments also induced rearrangement of the microtubular patterns with reversible depolymerization of microtubules. Acid phosphatase activity was significantly higher both in vitamin A-treated osteoclasts and in their media. When [3H]proline-labeled bone particles were added to the retinoid-treated osteoclasts, the release of [3H]proline was increased significantly compared to controls. These results suggest that the two vitamin A metabolites cause several modifications of the metabolic status of isolated osteoclasts that result in augmented rates of bone resorption.

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Published date: April 1988
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 360847
URI: https://eprints.soton.ac.uk/id/eprint/360847
ISSN: 0884-0431
PURE UUID: 19ab5f2d-11ec-4306-ac00-35e0d128ab9f
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 08 Jan 2014 15:38
Last modified: 06 Jun 2018 12:53

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