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Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption

Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption
Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption
Giant cell tumors of bone are common but unusual tumors that are comprised of multiple cell types. Most attention has been focused on the giant cells, which resemble osteoclasts morphologically and functionally. This study examines the properties of a cell line derived from mononuclear cells from one of these tumors, since it appears likely that these cells may be able to influence the activities of cells with the osteoclast phenotype. This cell line, C433, has the following characteristics: (1) it represents undifferentiated cells, not recognized by any known antigenic markers for leukocytes; (2) it contains tartrate-resistant acid phosphatase; (3) it responds to the osteotropic factors 1,25 dihydroxyvitamin D3, insulin-like growth factor I and II, but not to parathyroid hormone; (4) it forms sarcomas in nude mice; and (5) it produces an activity that stimulates isolated avian and rat osteoclasts to resorb bone. This cell line may be useful in examining interactions between osteoclasts and accessory cells involved in bone resorption.
0009-921X
229-241
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, G.J.
226b77a6-0299-4ba3-8093-73a59b3941f3
Kirchen, M.
4229df4b-c41c-4b1e-a7a4-23c5025e3510
Garcia, C.
c4feb44f-4d90-4f26-be1f-4375ed5ba48f
Gallwitz, W.E.
8d2012cd-2975-457f-824a-72f478c7c2e7
Chavez, J.
978b76ee-41a5-4136-8c32-cc62042c5c10
Mundy, G.R.
921d7037-eb6c-48bb-97a0-52d2647ccfda
Bonewald, L.F.
eba5037e-a9b4-43b7-b262-55e0d41ede2d
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, G.J.
226b77a6-0299-4ba3-8093-73a59b3941f3
Kirchen, M.
4229df4b-c41c-4b1e-a7a4-23c5025e3510
Garcia, C.
c4feb44f-4d90-4f26-be1f-4375ed5ba48f
Gallwitz, W.E.
8d2012cd-2975-457f-824a-72f478c7c2e7
Chavez, J.
978b76ee-41a5-4136-8c32-cc62042c5c10
Mundy, G.R.
921d7037-eb6c-48bb-97a0-52d2647ccfda
Bonewald, L.F.
eba5037e-a9b4-43b7-b262-55e0d41ede2d

Oreffo, R.O., Marshall, G.J., Kirchen, M., Garcia, C., Gallwitz, W.E., Chavez, J., Mundy, G.R. and Bonewald, L.F. (1993) Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption. Clinical Orthopaedics and Related Research, 296, 229-241. (PMID:8222432)

Record type: Article

Abstract

Giant cell tumors of bone are common but unusual tumors that are comprised of multiple cell types. Most attention has been focused on the giant cells, which resemble osteoclasts morphologically and functionally. This study examines the properties of a cell line derived from mononuclear cells from one of these tumors, since it appears likely that these cells may be able to influence the activities of cells with the osteoclast phenotype. This cell line, C433, has the following characteristics: (1) it represents undifferentiated cells, not recognized by any known antigenic markers for leukocytes; (2) it contains tartrate-resistant acid phosphatase; (3) it responds to the osteotropic factors 1,25 dihydroxyvitamin D3, insulin-like growth factor I and II, but not to parathyroid hormone; (4) it forms sarcomas in nude mice; and (5) it produces an activity that stimulates isolated avian and rat osteoclasts to resorb bone. This cell line may be useful in examining interactions between osteoclasts and accessory cells involved in bone resorption.

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Published date: November 1993
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 360855
URI: https://eprints.soton.ac.uk/id/eprint/360855
ISSN: 0009-921X
PURE UUID: e268acc7-b008-4046-ab15-aa5d166cf8e2
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 08 Jan 2014 16:59
Last modified: 06 Jun 2018 12:53

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Contributors

Author: R.O. Oreffo ORCID iD
Author: G.J. Marshall
Author: M. Kirchen
Author: C. Garcia
Author: W.E. Gallwitz
Author: J. Chavez
Author: G.R. Mundy
Author: L.F. Bonewald

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