Effects of beta mercaptoethanol on the proliferation and differentiation of human osteoprogenitor cells.
Effects of beta mercaptoethanol on the proliferation and differentiation of human osteoprogenitor cells.
Antioxidants are known to influence metabolism and promote cell survival in a number of cell culture systems. However, their effects on the modulation of bone cell differentiation in vitro are not clearly defined. In the present studies we have investigated the effects of beta-mercaptoethanol (beta ME) and ascorbate alone and in combination on human osteoprogenitors derived from bone marrow fibroblasts. In primary marrow cultures, beta ME stimulated colony formation (2-fold), alkaline phosphatase activity (3.5-fold) and, increased DNA synthesis (8-fold) after 21 days. Cell proliferation was increased significantly by beta ME during the first 4 days of a 10-day culture period, indicating stimulation of marrow osteoprogenitor proliferation. Ascorbate did not significantly augment the effects of beta ME in primary cultures or long-term cultures of passaged bone marrow fibroblasts. These findings indicate a potential beneficial role for beta ME addition for the optimal maintenance of colony formation, cell proliferation and differentiation of marrow osteoprogenitor cells in primary human bone marrow fibroblast cultures.
419-425
Inui, K.
706fd071-39ba-4fed-a641-f30a75c8e5ce
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
July 1997
Inui, K.
706fd071-39ba-4fed-a641-f30a75c8e5ce
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Inui, K., Oreffo, R.O. and Triffitt, J.T.
(1997)
Effects of beta mercaptoethanol on the proliferation and differentiation of human osteoprogenitor cells.
Cell Biology International, 21 (7), .
(PMID:9313342)
Abstract
Antioxidants are known to influence metabolism and promote cell survival in a number of cell culture systems. However, their effects on the modulation of bone cell differentiation in vitro are not clearly defined. In the present studies we have investigated the effects of beta-mercaptoethanol (beta ME) and ascorbate alone and in combination on human osteoprogenitors derived from bone marrow fibroblasts. In primary marrow cultures, beta ME stimulated colony formation (2-fold), alkaline phosphatase activity (3.5-fold) and, increased DNA synthesis (8-fold) after 21 days. Cell proliferation was increased significantly by beta ME during the first 4 days of a 10-day culture period, indicating stimulation of marrow osteoprogenitor proliferation. Ascorbate did not significantly augment the effects of beta ME in primary cultures or long-term cultures of passaged bone marrow fibroblasts. These findings indicate a potential beneficial role for beta ME addition for the optimal maintenance of colony formation, cell proliferation and differentiation of marrow osteoprogenitor cells in primary human bone marrow fibroblast cultures.
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Published date: July 1997
Organisations:
Human Development & Health
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Local EPrints ID: 360858
URI: http://eprints.soton.ac.uk/id/eprint/360858
ISSN: 1065-6995
PURE UUID: a0138a4b-318c-4a2c-bca5-36918dde1e6a
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Date deposited: 08 Jan 2014 17:11
Last modified: 08 Jan 2022 02:51
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Author:
K. Inui
Author:
J.T. Triffitt
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