The University of Southampton
University of Southampton Institutional Repository

Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures

Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures
Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures
Knowledge of the controlling mechanisms of human osteoprogenitor cell differentiation has important implications for understanding bone turnover. The in vitro differentiation of human bone marrow fibroblasts into adipogenic and osteogenic cells and the interaction of 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) and dexamethasone in this process has been investigated together with the effects of human serum. Marrow fibroblasts cultured in human serum and dexamethasone for 28 days, generated lipid containing cells as confirmed by morphology, Oil red O staining and immunocytochemistry using antiserum to the adipocyte-specific protein, adipocyte P2 (aP2). In cultures containing 1,25(OH)2D3 and dexamethasone, adipogenesis was stimulated within 21 days. Osteocalcin expression, as assessed by in situ hybridization, was dependent on the presence of 1,25(OH)2D3 and was decreased in cultures treated with dexamethasone. Northern analysis confirmed the decrease in osteocalcin expression and increase in lipoprotein lipase expression with the appearance of the adipogenic phenotype in these cultures. Marrow cultures maintained for 14 days in human serum and osteotropic agents before switching to fetal calf serum indicated the continuous requirement of human serum in these cultures for adipogenesis. These results demonstrate that human serum contains factors that exert dramatic effects on human bone marrow cell differentiation to augment the osteogenic and adipogenic activity of 1,25(OH)2D3 and dexamethasone.
0171-9335
251-261
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Virdi, A.S.
71654d0a-5481-4ff8-86d8-1c3d9c0aafd7
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Virdi, A.S.
71654d0a-5481-4ff8-86d8-1c3d9c0aafd7
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9

Oreffo, R.O., Virdi, A.S. and Triffitt, J.T. (1997) Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures. European Journal of Cell Biology, 74 (3), 251-261. (PMID:9402473)

Record type: Article

Abstract

Knowledge of the controlling mechanisms of human osteoprogenitor cell differentiation has important implications for understanding bone turnover. The in vitro differentiation of human bone marrow fibroblasts into adipogenic and osteogenic cells and the interaction of 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) and dexamethasone in this process has been investigated together with the effects of human serum. Marrow fibroblasts cultured in human serum and dexamethasone for 28 days, generated lipid containing cells as confirmed by morphology, Oil red O staining and immunocytochemistry using antiserum to the adipocyte-specific protein, adipocyte P2 (aP2). In cultures containing 1,25(OH)2D3 and dexamethasone, adipogenesis was stimulated within 21 days. Osteocalcin expression, as assessed by in situ hybridization, was dependent on the presence of 1,25(OH)2D3 and was decreased in cultures treated with dexamethasone. Northern analysis confirmed the decrease in osteocalcin expression and increase in lipoprotein lipase expression with the appearance of the adipogenic phenotype in these cultures. Marrow cultures maintained for 14 days in human serum and osteotropic agents before switching to fetal calf serum indicated the continuous requirement of human serum in these cultures for adipogenesis. These results demonstrate that human serum contains factors that exert dramatic effects on human bone marrow cell differentiation to augment the osteogenic and adipogenic activity of 1,25(OH)2D3 and dexamethasone.

This record has no associated files available for download.

More information

Published date: November 1997
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 360859
URI: http://eprints.soton.ac.uk/id/eprint/360859
ISSN: 0171-9335
PURE UUID: dd492f5d-020e-49be-a8e2-dfb75bf45065
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 08 Jan 2014 17:12
Last modified: 09 Jan 2022 03:02

Export record

Contributors

Author: R.O. Oreffo ORCID iD
Author: A.S. Virdi
Author: J.T. Triffitt

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×