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Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements

Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements
Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements
Materials that augment bone cell proliferation and osteogenic activity have important therapeutic implications for bone regeneration and for use in skeletal reconstruction and joint replacement. We have studied the growth and interactions of human bone marrow cells on a variety of new cement composites in vitro. These cement materials are composed of calcium-deficient hydroxyapatites, carbonated apatite and amorphous calcium phosphate. Cell proliferation was significantly reduced and cell differentiation increased in the presence of these cements compared with cells cultured on tissue culture plastic. Alkaline phosphatase, one of the markers of the osteoblast phenotype, was dramatically stimulated by 3 of the 4 cements examined between day 4 and day 10, above levels observed following culture of human osteoblasts on plastic alone. Photomicroscopic examination demonstrated growth and close integration of bone marrow cells and 3 of the composites. Longer term marrow cultures (15 day) on the cements confirmed the stimulation of cell differentiation over proliferation. From these studies, enhanced osteoblastic differentiation was observed on a 70% carbonated apatite, which has a composition similar to bone mineral, whereas, cell toxicity was observed on cells grown on amorphous calcium phosphate. This in vitro culture system demonstrates the use of human bone marrow cells for the potential evaluation of new biomaterials and the development of a novel carbonated apatite that may be of potential use in orthopaedic implants
0142-9612
1845-1854
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Driessens, F.C.
97cddc35-fc38-461b-955f-551ef940743f
Planell, J.A.
38a45d41-f09d-49b2-9bdd-e928709507d8
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Driessens, F.C.
97cddc35-fc38-461b-955f-551ef940743f
Planell, J.A.
38a45d41-f09d-49b2-9bdd-e928709507d8
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9

Oreffo, R.O., Driessens, F.C., Planell, J.A. and Triffitt, J.T. (1998) Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements. Biomaterials, 19 (20), 1845-1854. (doi:10.1016/S0142-9612(98)00084-2). (PMID:9855185)

Record type: Article

Abstract

Materials that augment bone cell proliferation and osteogenic activity have important therapeutic implications for bone regeneration and for use in skeletal reconstruction and joint replacement. We have studied the growth and interactions of human bone marrow cells on a variety of new cement composites in vitro. These cement materials are composed of calcium-deficient hydroxyapatites, carbonated apatite and amorphous calcium phosphate. Cell proliferation was significantly reduced and cell differentiation increased in the presence of these cements compared with cells cultured on tissue culture plastic. Alkaline phosphatase, one of the markers of the osteoblast phenotype, was dramatically stimulated by 3 of the 4 cements examined between day 4 and day 10, above levels observed following culture of human osteoblasts on plastic alone. Photomicroscopic examination demonstrated growth and close integration of bone marrow cells and 3 of the composites. Longer term marrow cultures (15 day) on the cements confirmed the stimulation of cell differentiation over proliferation. From these studies, enhanced osteoblastic differentiation was observed on a 70% carbonated apatite, which has a composition similar to bone mineral, whereas, cell toxicity was observed on cells grown on amorphous calcium phosphate. This in vitro culture system demonstrates the use of human bone marrow cells for the potential evaluation of new biomaterials and the development of a novel carbonated apatite that may be of potential use in orthopaedic implants

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Published date: October 1998
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 360865
URI: https://eprints.soton.ac.uk/id/eprint/360865
ISSN: 0142-9612
PURE UUID: 35ab8ef0-4e57-4ebe-a18a-efb1718bf109
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 09 Jan 2014 11:03
Last modified: 06 Jun 2018 12:53

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