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Effects of TGF? and bFGF on the differentiation of human bone marrow stromal fibroblasts

Effects of TGF? and bFGF on the differentiation of human bone marrow stromal fibroblasts
Effects of TGF? and bFGF on the differentiation of human bone marrow stromal fibroblasts
Adipocytes and osteoblasts have common origins from fibroblastic stem cells. Consequently, modulation of the processes of adipogenesis and osteogenesis has implications for the possible treatment of metabolic bone diseases, such as osteoporosis, in which medullary fat accumulates and trabecular bone volume decreases. It is likely that the balance between these two systems is affected by particular endogenous growth factors which are known to affect bone metabolism. We have therefore investigated the effects of transforming growth factor beta (TGF?), basic fibroblast growth factor (bFGF) and dexamethasone (Dex) on cultured human bone marrow (HBM) fibroblastic cells to observe the effects on adipogenesis and osteogenesis. In the absence of fetal calf serum (FCS), TGF? caused a dose-dependent increase in cell growth and alkaline phosphatase activity (AP); however, in the presence of FCS growth was inhibited at high concentrations and AP unaffected. TGF? increased matrix proteoglycan and collagen synthesis. bFGF inhibited AP and increased colony number and size, while Dex treatment increased AP activity and colony number, and both factors in combination resulted in an additive increase in growth. Dex-induced adipocyte formation was accelerated but not increased by bFGF. A significant inhibition of adipogenesis by TGF? was observed within 7 days. These results demonstrate the importance of biological factors known to be involved in bone remodelling in the regulation of osteogenesis and adipogenesis.
TGF?, bFGF, human, bone marrow, fibroblasts
1065-6995
185-194
Locklin, R.M.
879f95db-0fc5-4353-a81b-9aec5d24bc5e
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9
Locklin, R.M.
879f95db-0fc5-4353-a81b-9aec5d24bc5e
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Triffitt, J.T.
06d3019a-06e6-4abd-9e73-f073d621e1f9

Locklin, R.M., Oreffo, R.O. and Triffitt, J.T. (1999) Effects of TGF? and bFGF on the differentiation of human bone marrow stromal fibroblasts. Cell Biology International, 23 (3), 185-194. (PMID:10562439)

Record type: Article

Abstract

Adipocytes and osteoblasts have common origins from fibroblastic stem cells. Consequently, modulation of the processes of adipogenesis and osteogenesis has implications for the possible treatment of metabolic bone diseases, such as osteoporosis, in which medullary fat accumulates and trabecular bone volume decreases. It is likely that the balance between these two systems is affected by particular endogenous growth factors which are known to affect bone metabolism. We have therefore investigated the effects of transforming growth factor beta (TGF?), basic fibroblast growth factor (bFGF) and dexamethasone (Dex) on cultured human bone marrow (HBM) fibroblastic cells to observe the effects on adipogenesis and osteogenesis. In the absence of fetal calf serum (FCS), TGF? caused a dose-dependent increase in cell growth and alkaline phosphatase activity (AP); however, in the presence of FCS growth was inhibited at high concentrations and AP unaffected. TGF? increased matrix proteoglycan and collagen synthesis. bFGF inhibited AP and increased colony number and size, while Dex treatment increased AP activity and colony number, and both factors in combination resulted in an additive increase in growth. Dex-induced adipocyte formation was accelerated but not increased by bFGF. A significant inhibition of adipogenesis by TGF? was observed within 7 days. These results demonstrate the importance of biological factors known to be involved in bone remodelling in the regulation of osteogenesis and adipogenesis.

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More information

Published date: 1999
Keywords: TGF?, bFGF, human, bone marrow, fibroblasts
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 360867
URI: http://eprints.soton.ac.uk/id/eprint/360867
ISSN: 1065-6995
PURE UUID: 1e01e16a-089a-450f-84c8-6ab98ce56e4c
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 09 Jan 2014 11:25
Last modified: 05 Nov 2019 01:56

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Contributors

Author: R.M. Locklin
Author: R.O. Oreffo ORCID iD
Author: J.T. Triffitt

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