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Soft-tissue and phase-contrast imaging at the Swiss Light Source

Soft-tissue and phase-contrast imaging at the Swiss Light Source
Soft-tissue and phase-contrast imaging at the Swiss Light Source
Recent results show that bone vasculature is a major contributor to local tissue porosity, and therefore can be directly linked to the mechanical properties of bone tissue. With the advent of third generation synchrotron radiation (SR) sources, micro-computed tomography (?CT) with resolutions in the order of 1 ?m and better has become feasible. This technique has been employed frequently to analyze trabecular architecture and local bone tissue properties, i.e. the hard or mineralized bone tissue. Nevertheless, less is known about the soft tissues in bone, mainly due to inadequate imaging capabilities. Here, we discuss three different methods and applications to visualize soft tissues. The first approach is referred to as negative imaging. In this case the material around the soft tissue provides the absorption contrast necessary for X-ray based tomography. Bone vasculature from two different mouse strains was investigated and compared qualitatively. Differences were observed in terms of local vessel number and vessel orientation. The second technique represents corrosion casting, which is principally adapted for imaging of vascular systems. The technique of corrosion casting has already been applied successfully at the Swiss Light Source. Using the technology we were able to show that pathological features reminiscent of Alzheimer"s disease could be distinguished in the brain vasculature of APP transgenic mice. The third technique discussed here is phase contrast imaging exploiting the high degree of coherence of third generation synchrotron light sources, which provide the necessary physical conditions for phase contrast. The in-line approach followed here for phase contrast retrieval is a modification of the Gerchberg-Saxton-Fienup type. Several measurements and theoretical thoughts concerning phase contrast imaging are presented, including mathematical phase retrieval. Although up-to-now only phase images have been computed, the approach is now ready to retrieve the phase for a large number of angular positions of the specimen allowing application of holotomography, which is the three-dimensional reconstruction of phase images.
light sources, phase contrast, tissues, bone, corrosion, diseases and disorders, brain, phase retrieval, synchrotron radiation, microcomputed tomography
0277-786X
281-291
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Mohan, Nishant
24e12ba1-b6a4-41a7-b4eb-13865f6bd8b0
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Mohan, Nishant
24e12ba1-b6a4-41a7-b4eb-13865f6bd8b0
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Schneider, Philipp, Mohan, Nishant, Stampanoni, Marco and Müller, Ralph (2004) Soft-tissue and phase-contrast imaging at the Swiss Light Source. [in special issue: Medical Imaging 2004: Physics of Medical Imaging] Proceedings of SPIE, 5368, 281-291. (doi:10.1117/12.533716).

Record type: Article

Abstract

Recent results show that bone vasculature is a major contributor to local tissue porosity, and therefore can be directly linked to the mechanical properties of bone tissue. With the advent of third generation synchrotron radiation (SR) sources, micro-computed tomography (?CT) with resolutions in the order of 1 ?m and better has become feasible. This technique has been employed frequently to analyze trabecular architecture and local bone tissue properties, i.e. the hard or mineralized bone tissue. Nevertheless, less is known about the soft tissues in bone, mainly due to inadequate imaging capabilities. Here, we discuss three different methods and applications to visualize soft tissues. The first approach is referred to as negative imaging. In this case the material around the soft tissue provides the absorption contrast necessary for X-ray based tomography. Bone vasculature from two different mouse strains was investigated and compared qualitatively. Differences were observed in terms of local vessel number and vessel orientation. The second technique represents corrosion casting, which is principally adapted for imaging of vascular systems. The technique of corrosion casting has already been applied successfully at the Swiss Light Source. Using the technology we were able to show that pathological features reminiscent of Alzheimer"s disease could be distinguished in the brain vasculature of APP transgenic mice. The third technique discussed here is phase contrast imaging exploiting the high degree of coherence of third generation synchrotron light sources, which provide the necessary physical conditions for phase contrast. The in-line approach followed here for phase contrast retrieval is a modification of the Gerchberg-Saxton-Fienup type. Several measurements and theoretical thoughts concerning phase contrast imaging are presented, including mathematical phase retrieval. Although up-to-now only phase images have been computed, the approach is now ready to retrieve the phase for a large number of angular positions of the specimen allowing application of holotomography, which is the three-dimensional reconstruction of phase images.

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More information

Published date: 6 May 2004
Keywords: light sources, phase contrast, tissues, bone, corrosion, diseases and disorders, brain, phase retrieval, synchrotron radiation, microcomputed tomography
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 361052
URI: http://eprints.soton.ac.uk/id/eprint/361052
ISSN: 0277-786X
PURE UUID: b98dda98-1790-4f4b-aaed-02fef5061a3a
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

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Date deposited: 14 Jan 2014 11:52
Last modified: 10 Dec 2019 01:34

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Contributors

Author: Nishant Mohan
Author: Marco Stampanoni
Author: Ralph Müller

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