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Assessment of murine bone ultrastructure using synchrotron light: towards nano-computed tomography

Assessment of murine bone ultrastructure using synchrotron light: towards nano-computed tomography
Assessment of murine bone ultrastructure using synchrotron light: towards nano-computed tomography
To describe the different aspects of bone quality, we follow a hierarchical approach and assess bone tissue properties in different regimes of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes we developed different synchrotron radiation (SR) based computed-tomography (CT) methods to assess murine bone ultrastructure. In a first step, a tubular system and the osteocyte lacunar system within murine cortical bone have been established as novel ultrastructural quantitative traits. Results in two mouse strains showed that morphometry of these quantitative traits was dependent on strain and partially on gender, and that their scaling behavior with bone size was fundamentally different. In a second step, we explored bone competence on an ultrastructural level and related our findings to the two ultrastructural quantitative traits introduced before. We showed that SR CT imaging is a powerful tool to investigate the initiation and propagation of microcracks, which may alter bone quality and may lead to increased fracture risk by means of microdamage accumulation. In summary, investigation of ultrastructural bone tissue properties will eventually lead to a better understanding of bone quality and its relative contribution to bone competence.
bone, tomography, computed tomography, synchrotron radiation
0277-786X
Schneider, Philipp
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Voide, Romain
8859d4cc-c065-4034-b350-0538997ce8fe
Stauber, Martin
f69dbdc0-ebca-41eb-aab7-0679e6b78874
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Donahue, Leah Rae
a3e059f6-5339-43e1-89cc-5d8af96e800b
Wyss, Peter
37835676-2f92-4df7-bc35-97b2c80c1104
Sennhauser, Urs
3c0e14aa-da0a-48da-bb7b-c65401b1d01b
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Bonse, Ulrich
0ed37e40-03f2-4a26-aa8c-6959f6c18259
Bonse, Ulrich
0ed37e40-03f2-4a26-aa8c-6959f6c18259
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Voide, Romain
8859d4cc-c065-4034-b350-0538997ce8fe
Stauber, Martin
f69dbdc0-ebca-41eb-aab7-0679e6b78874
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Donahue, Leah Rae
a3e059f6-5339-43e1-89cc-5d8af96e800b
Wyss, Peter
37835676-2f92-4df7-bc35-97b2c80c1104
Sennhauser, Urs
3c0e14aa-da0a-48da-bb7b-c65401b1d01b
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Schneider, Philipp, Voide, Romain, Stauber, Martin, Stampanoni, Marco, Donahue, Leah Rae, Wyss, Peter, Sennhauser, Urs and Müller, Ralph , Bonse, Ulrich (ed.) (2006) Assessment of murine bone ultrastructure using synchrotron light: towards nano-computed tomography. [in special issue: Developments in X-Ray Tomography V] Proceedings of SPIE, 63180C. (doi:10.1117/12.679427).

Record type: Article

Abstract

To describe the different aspects of bone quality, we follow a hierarchical approach and assess bone tissue properties in different regimes of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes we developed different synchrotron radiation (SR) based computed-tomography (CT) methods to assess murine bone ultrastructure. In a first step, a tubular system and the osteocyte lacunar system within murine cortical bone have been established as novel ultrastructural quantitative traits. Results in two mouse strains showed that morphometry of these quantitative traits was dependent on strain and partially on gender, and that their scaling behavior with bone size was fundamentally different. In a second step, we explored bone competence on an ultrastructural level and related our findings to the two ultrastructural quantitative traits introduced before. We showed that SR CT imaging is a powerful tool to investigate the initiation and propagation of microcracks, which may alter bone quality and may lead to increased fracture risk by means of microdamage accumulation. In summary, investigation of ultrastructural bone tissue properties will eventually lead to a better understanding of bone quality and its relative contribution to bone competence.

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More information

Published date: 7 September 2006
Keywords: bone, tomography, computed tomography, synchrotron radiation
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 361056
URI: http://eprints.soton.ac.uk/id/eprint/361056
ISSN: 0277-786X
PURE UUID: cecae35c-ac30-436b-8a3d-d23ed6980d7a
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

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Date deposited: 14 Jan 2014 12:32
Last modified: 03 Dec 2019 01:35

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Contributors

Editor: Ulrich Bonse
Author: Romain Voide
Author: Martin Stauber
Author: Marco Stampanoni
Author: Leah Rae Donahue
Author: Peter Wyss
Author: Urs Sennhauser
Author: Ralph Müller

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