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Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT

Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT
Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT
Nondestructive SR-based ?CT and nano-CT methods have been designed for 3D quantification and morphometric analysis of ultrastructural phenotypes within murine cortical bone, namely the canal network and the osteocyte lacunar system. Results in two different mouse strains, C57BL/6J-Ghrhrlit/J and C3.B6-Ghrhrlit/J, showed that the cannular and lacunar morphometry and their bone mechanics were fundamentally different.

Introduction: to describe the different aspects of bone quality, we followed a hierarchical approach and assessed bone tissue properties in different regimens of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes, we developed different synchrotron radiation (SR)-based CT methods to assess ultrastructural phenotypes of murine bone.

Materials and methods: the femoral mid-diaphyses of 12 C57BL/6J-Ghrhrlit/J (B6-lit/lit) and 12 homozygous mutants C3.B6-Ghrhrlit/J (C3.B6-lit/lit) were measured with global SR ?CT and local SR nano-CT (nCT) at nominal resolutions ranging from 3.5 ?m to 700 nm, respectively. For volumetric quantification, morphometric indices were determined for the cortical bone, the canal network, and the osteocyte lacunar system using negative imaging. Moreover, the biomechanics of B6-lit/lit and C3.B6-lit/lit mice was determined by three-point bending.

Results: the femoral mid-diaphysis of C3.B6-lit/lit was larger compared with B6-lit/lit mice. On an ultrastructural level, the cannular indices for C3.B6-lit/lit were generally bigger in comparison with B6-lit/lit mice. Accordingly, we derived and showed a scaling rule, saying that overall cannular indices scaled with bone size, whereas indices describing basic elements of cannular and lacunar morphometry did not. Although in C3.B6-lit/lit, the mean canal volume was larger than in B6-lit/lit, canal number density was proportionally smaller in C3.B6-lit/lit, so that lacuna volume density was found to be constant and therefore independent of mouse strain and sex. The mechanical properties in C3.B6-lit/lit were generally improved compared with B6-lit/lit specimens. For C3.B6-lit/lit, we observed a sex specificity of the mechanical parameters, which could not be explained by bone morphometry on an organ level. However, there is evidence that for C3.B6-lit/lit, the larger cortical bone mass is counterbalanced or even outweighed by the larger canal network in the female mice.

Conclusions: we established a strategy to subdivide murine intracortical porosity into ultrastructural phenotypes, namely the canal network and the osteocyte lacunar system. Nondestructive global and local SR-based CT methods have been designed for 3D quantification and subsequent morphometric analysis of these phenotypes. Results in the two different mouse strains C57BL/6J-Ghrhrlit/J and C3.B6-Ghrhrlit/J showed that the cannular and lacunar morphometry and the biomechanical properties were fundamentally different
0884-0431
1557-1570
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Stauber, Martin
f69dbdc0-ebca-41eb-aab7-0679e6b78874
Voide, Romain
8859d4cc-c065-4034-b350-0538997ce8fe
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Donahue, Leah Rae
a3e059f6-5339-43e1-89cc-5d8af96e800b
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Stauber, Martin
f69dbdc0-ebca-41eb-aab7-0679e6b78874
Voide, Romain
8859d4cc-c065-4034-b350-0538997ce8fe
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Donahue, Leah Rae
a3e059f6-5339-43e1-89cc-5d8af96e800b
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Schneider, Philipp, Stauber, Martin, Voide, Romain, Stampanoni, Marco, Donahue, Leah Rae and Müller, Ralph (2007) Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT. Journal of Bone and Mineral Research, 22 (10), 1557-1570. (doi:10.1359/JBMR.070703). (PMID:17605631)

Record type: Article

Abstract

Nondestructive SR-based ?CT and nano-CT methods have been designed for 3D quantification and morphometric analysis of ultrastructural phenotypes within murine cortical bone, namely the canal network and the osteocyte lacunar system. Results in two different mouse strains, C57BL/6J-Ghrhrlit/J and C3.B6-Ghrhrlit/J, showed that the cannular and lacunar morphometry and their bone mechanics were fundamentally different.

Introduction: to describe the different aspects of bone quality, we followed a hierarchical approach and assessed bone tissue properties in different regimens of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes, we developed different synchrotron radiation (SR)-based CT methods to assess ultrastructural phenotypes of murine bone.

Materials and methods: the femoral mid-diaphyses of 12 C57BL/6J-Ghrhrlit/J (B6-lit/lit) and 12 homozygous mutants C3.B6-Ghrhrlit/J (C3.B6-lit/lit) were measured with global SR ?CT and local SR nano-CT (nCT) at nominal resolutions ranging from 3.5 ?m to 700 nm, respectively. For volumetric quantification, morphometric indices were determined for the cortical bone, the canal network, and the osteocyte lacunar system using negative imaging. Moreover, the biomechanics of B6-lit/lit and C3.B6-lit/lit mice was determined by three-point bending.

Results: the femoral mid-diaphysis of C3.B6-lit/lit was larger compared with B6-lit/lit mice. On an ultrastructural level, the cannular indices for C3.B6-lit/lit were generally bigger in comparison with B6-lit/lit mice. Accordingly, we derived and showed a scaling rule, saying that overall cannular indices scaled with bone size, whereas indices describing basic elements of cannular and lacunar morphometry did not. Although in C3.B6-lit/lit, the mean canal volume was larger than in B6-lit/lit, canal number density was proportionally smaller in C3.B6-lit/lit, so that lacuna volume density was found to be constant and therefore independent of mouse strain and sex. The mechanical properties in C3.B6-lit/lit were generally improved compared with B6-lit/lit specimens. For C3.B6-lit/lit, we observed a sex specificity of the mechanical parameters, which could not be explained by bone morphometry on an organ level. However, there is evidence that for C3.B6-lit/lit, the larger cortical bone mass is counterbalanced or even outweighed by the larger canal network in the female mice.

Conclusions: we established a strategy to subdivide murine intracortical porosity into ultrastructural phenotypes, namely the canal network and the osteocyte lacunar system. Nondestructive global and local SR-based CT methods have been designed for 3D quantification and subsequent morphometric analysis of these phenotypes. Results in the two different mouse strains C57BL/6J-Ghrhrlit/J and C3.B6-Ghrhrlit/J showed that the cannular and lacunar morphometry and the biomechanical properties were fundamentally different

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Published date: 2 July 2007
Organisations: Faculty of Engineering and the Environment

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Local EPrints ID: 361059
URI: http://eprints.soton.ac.uk/id/eprint/361059
ISSN: 0884-0431
PURE UUID: 2273a93b-8827-41f4-92f8-88f8e2d60b1e
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

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Date deposited: 13 Jan 2014 11:17
Last modified: 17 Dec 2019 01:36

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Contributors

Author: Martin Stauber
Author: Romain Voide
Author: Marco Stampanoni
Author: Leah Rae Donahue
Author: Ralph Müller

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