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Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica

Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica
Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica
Recent evidence suggests a close functional relationship between osteogenesis and angiogenesis as well as between bone remodeling and bone vascularization. Consequently, there is a need for visual inspection and quantitative analysis of the bone vasculature. We therefore adapted and implemented two different vascular corrosion casting (VCC) protocols using a polyurethane-based casting resin in mice for a true three-dimensional (3D), direct, and simultaneous measurement of bone tissue and vascular morphology by micro-computed tomography (?CT). For assessment of vascular replicas at the level of capillaries, a vascular contrast perfusion (VCP) protocol was devised using a contrast modality based on a barium sulfate suspension in conjunction with synchrotron radiation (SR) ?CT. The vascular morphology quantified using the VCP protocol was compared quantitatively with the results of a previously established method, where the vascular network of cortical bone was derived indirectly from cortical porosity. The presented VCC and VCP protocols have the potential of serving as a valuable method for concomitant 3D quantitative morphometry of the bone tissue and its vasculature
1059-910X
690-701
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Krucker, Thomas
c2e8925b-2bdc-4d43-bc81-a0d8aadb97b8
Meyer, Eric
52c7ac3b-632b-44fb-a53a-ca834aae421f
Ulmann-Schuler, Alexandra
92dc5ddc-1023-4efb-b4b9-eac64d75d39b
Weber, Bruno
cbb56677-09f4-404e-bea4-485b0bfe0d01
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Krucker, Thomas
c2e8925b-2bdc-4d43-bc81-a0d8aadb97b8
Meyer, Eric
52c7ac3b-632b-44fb-a53a-ca834aae421f
Ulmann-Schuler, Alexandra
92dc5ddc-1023-4efb-b4b9-eac64d75d39b
Weber, Bruno
cbb56677-09f4-404e-bea4-485b0bfe0d01
Stampanoni, Marco
bfedb3b0-01e8-4e1b-9163-41295b4ceeb1
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Schneider, Philipp, Krucker, Thomas, Meyer, Eric, Ulmann-Schuler, Alexandra, Weber, Bruno, Stampanoni, Marco and Müller, Ralph (2009) Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica Microscopy Research and Technique, 72, (9), pp. 690-701. (doi:10.1002/jemt.20720).

Record type: Article

Abstract

Recent evidence suggests a close functional relationship between osteogenesis and angiogenesis as well as between bone remodeling and bone vascularization. Consequently, there is a need for visual inspection and quantitative analysis of the bone vasculature. We therefore adapted and implemented two different vascular corrosion casting (VCC) protocols using a polyurethane-based casting resin in mice for a true three-dimensional (3D), direct, and simultaneous measurement of bone tissue and vascular morphology by micro-computed tomography (?CT). For assessment of vascular replicas at the level of capillaries, a vascular contrast perfusion (VCP) protocol was devised using a contrast modality based on a barium sulfate suspension in conjunction with synchrotron radiation (SR) ?CT. The vascular morphology quantified using the VCP protocol was compared quantitatively with the results of a previously established method, where the vascular network of cortical bone was derived indirectly from cortical porosity. The presented VCC and VCP protocols have the potential of serving as a valuable method for concomitant 3D quantitative morphometry of the bone tissue and its vasculature

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Published date: 9 April 2009
Organisations: Faculty of Engineering and the Environment

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Local EPrints ID: 361065
URI: http://eprints.soton.ac.uk/id/eprint/361065
ISSN: 1059-910X
PURE UUID: cf7d23ff-2232-4ebb-921a-8dee8aa28539
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

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Date deposited: 13 Jan 2014 11:44
Last modified: 18 Jul 2017 03:07

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Contributors

Author: Thomas Krucker
Author: Eric Meyer
Author: Alexandra Ulmann-Schuler
Author: Bruno Weber
Author: Marco Stampanoni
Author: Ralph Müller

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