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Studying osteocytes within their environment

Studying osteocytes within their environment
Studying osteocytes within their environment
It is widely hypothesized that osteocytes are the mechano-sensors residing in the bone's mineralized matrix which control load induced bone adaptation. Diving to their inaccessibility it has proved challenging to generate quantitative in vivo experimental data which supports this hypothesis. Recent advances in in situ imaging, both in non-living and living specimens, have provided new insights into the role of osteocytes in the skeleton. Combined with the retrieval of biochemical information from mechanically stimulated osteocytes using in vivo models, quantitative experimental data is now becoming available which is leading to a more accurate understanding of osteocyte function. With this in mind, here we review i) state of the art ex vivo imaging modalities which are able to precisely capture osteocyte structure in 3D, ii) live cell imaging techniques which are able to track structural morphology and cellular differentiation in both space and time, and iii) in vivo models which when combined with the latest biochemical assays and microfluidic imaging techniques can provide further insight on the biological function of osteocytes.
This article is part of a Special Issue entitled "The Osteocyte".
osteocytes, high resolution imaging, live cell imaging, microstructure, gene expression, in vivo models
8756-3282
285-295
Webster, Duncan J.
5eaa9b1a-04de-47f6-bbbc-5a2b00448a12
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Dallas, Sarah L.
d487a2e5-345a-4091-8fbd-bb0155c91198
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Webster, Duncan J.
5eaa9b1a-04de-47f6-bbbc-5a2b00448a12
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Dallas, Sarah L.
d487a2e5-345a-4091-8fbd-bb0155c91198
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Webster, Duncan J., Schneider, Philipp, Dallas, Sarah L. and Müller, Ralph (2013) Studying osteocytes within their environment. Bone, 54 (2), 285-295. (doi:10.1016/j.bone.2013.01.004).

Record type: Article

Abstract

It is widely hypothesized that osteocytes are the mechano-sensors residing in the bone's mineralized matrix which control load induced bone adaptation. Diving to their inaccessibility it has proved challenging to generate quantitative in vivo experimental data which supports this hypothesis. Recent advances in in situ imaging, both in non-living and living specimens, have provided new insights into the role of osteocytes in the skeleton. Combined with the retrieval of biochemical information from mechanically stimulated osteocytes using in vivo models, quantitative experimental data is now becoming available which is leading to a more accurate understanding of osteocyte function. With this in mind, here we review i) state of the art ex vivo imaging modalities which are able to precisely capture osteocyte structure in 3D, ii) live cell imaging techniques which are able to track structural morphology and cellular differentiation in both space and time, and iii) in vivo models which when combined with the latest biochemical assays and microfluidic imaging techniques can provide further insight on the biological function of osteocytes.
This article is part of a Special Issue entitled "The Osteocyte".

Full text not available from this repository.

More information

e-pub ahead of print date: 11 January 2013
Published date: June 2013
Keywords: osteocytes, high resolution imaging, live cell imaging, microstructure, gene expression, in vivo models
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 361075
URI: http://eprints.soton.ac.uk/id/eprint/361075
ISSN: 8756-3282
PURE UUID: 82bcf3d2-26d8-4eb1-abb7-155f805f32d0
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

Catalogue record

Date deposited: 14 Jan 2014 16:51
Last modified: 17 Dec 2019 01:36

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Contributors

Author: Duncan J. Webster
Author: Sarah L. Dallas
Author: Ralph Müller

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