The University of Southampton
University of Southampton Institutional Repository

Compartmentalized Calcium Signaling in Cilia Regulates Intraflagellar Transport

Compartmentalized Calcium Signaling in Cilia Regulates Intraflagellar Transport
Compartmentalized Calcium Signaling in Cilia Regulates Intraflagellar Transport
Intraflagellar transport (IFT) underpins many of the important cellular roles of cilia and flagella in signaling and motility [1,2,3,4]. The microtubule motors kinesin-2 and cytoplasmic dynein 1b drive IFT particles (protein complexes carrying ciliary component proteins) along the axoneme to facilitate the assembly and maintenance of cilia. IFT is regulated primarily by cargo loading onto the IFT particles, although evidence suggests that IFT particles also exhibit differential rates of movement [5,6,7]. Here we demonstrate that intraflagellar Ca2+ elevations act to directly regulate the movement of IFT particles. IFT-driven movement of adherent flagella membrane glycoproteins in the model alga Chlamydomonas enables flagella-mediated gliding motility [8,9,10]. We find that surface contact promotes the localized accumulation of IFT particles in Chlamydomonas flagella. Highly compartmentalized intraflagellar Ca2+ elevations initiate retrograde transport of paused IFT particles to modulate their accumulation. Gliding motility induces mechanosensitive intraflagellar Ca2+ elevations in trailing (dragging) flagella only, acting to specifically clear the accumulated microtubule motors from individual flagella and prevent a futile tug-of-war. Our results demonstrate that compartmentalized intraciliary Ca2+ signaling can regulate the movement of IFT particles and is therefore likely to play a central role in directing the movement and distribution of many ciliary proteins.
0960-9822
2311-2318
Collingridge, Peter
b7b8b73b-815d-48af-a637-c0b10f2b55b4
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a
Collingridge, Peter
b7b8b73b-815d-48af-a637-c0b10f2b55b4
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a

Collingridge, Peter, Brownlee, Colin and Wheeler, Glen L. (2013) Compartmentalized Calcium Signaling in Cilia Regulates Intraflagellar Transport. Current Biology, 23 (22), 2311-2318. (doi:10.1016/j.cub.2013.09.059).

Record type: Article

Abstract

Intraflagellar transport (IFT) underpins many of the important cellular roles of cilia and flagella in signaling and motility [1,2,3,4]. The microtubule motors kinesin-2 and cytoplasmic dynein 1b drive IFT particles (protein complexes carrying ciliary component proteins) along the axoneme to facilitate the assembly and maintenance of cilia. IFT is regulated primarily by cargo loading onto the IFT particles, although evidence suggests that IFT particles also exhibit differential rates of movement [5,6,7]. Here we demonstrate that intraflagellar Ca2+ elevations act to directly regulate the movement of IFT particles. IFT-driven movement of adherent flagella membrane glycoproteins in the model alga Chlamydomonas enables flagella-mediated gliding motility [8,9,10]. We find that surface contact promotes the localized accumulation of IFT particles in Chlamydomonas flagella. Highly compartmentalized intraflagellar Ca2+ elevations initiate retrograde transport of paused IFT particles to modulate their accumulation. Gliding motility induces mechanosensitive intraflagellar Ca2+ elevations in trailing (dragging) flagella only, acting to specifically clear the accumulated microtubule motors from individual flagella and prevent a futile tug-of-war. Our results demonstrate that compartmentalized intraciliary Ca2+ signaling can regulate the movement of IFT particles and is therefore likely to play a central role in directing the movement and distribution of many ciliary proteins.

This record has no associated files available for download.

More information

Published date: 7 November 2013
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 361124
URI: http://eprints.soton.ac.uk/id/eprint/361124
ISSN: 0960-9822
PURE UUID: 1aacb4cc-fb24-4c2d-ab8d-38d11b01828d

Catalogue record

Date deposited: 13 Jan 2014 16:42
Last modified: 14 Mar 2024 15:46

Export record

Altmetrics

Contributors

Author: Peter Collingridge
Author: Colin Brownlee
Author: Glen L. Wheeler

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×