The University of Southampton
University of Southampton Institutional Repository

UVA irradiation of human skin vasodilates arterial vasculature and lowers blood pressure independently of nitric oxide synthase

UVA irradiation of human skin vasodilates arterial vasculature and lowers blood pressure independently of nitric oxide synthase
UVA irradiation of human skin vasodilates arterial vasculature and lowers blood pressure independently of nitric oxide synthase
The incidence of hypertension and cardiovascular disease correlates with latitude and rises in winter. The molecular basis for this remains obscure. As nitric oxide (NO) metabolites are abundant in human skin we hypothesised that exposure to UVA may mobilise NO bioactivity into the circulation to exert beneficial cardiovascular effects independently of vitamin D. In 24 healthy volunteers irradiation of the skin with 2 Standard Erythemal Doses of UVA lowered BP, with concomitant decreases in circulating nitrate and rises in nitrite concentrations. Unexpectedly, acute dietary intervention aimed at modulating systemic nitrate availability had no effect on UV-induced hemodynamic changes, indicating that cardiovascular effects were not mediated via direct utilization of circulating nitrate. UVA irradiation of the forearm caused increased blood flow independently of NO-synthase activity, suggesting involvement of pre-formed cutaneous NO stores. Confocal fluorescence microscopy studies of human skin pre-labelled with the NO-imaging probe DAF2-DA revealed that UVA-induced NO release occurs in a NOS-independent, dose-dependent fashion, with the majority of the light-sensitive NO pool in the upper epidermis. Collectively, our data provide mechanistic insights into an important function of the skin in modulating systemic NO bioavailability which may account for the latitudinal and seasonal variations of BP and cardiovascular disease.Journal of Investigative Dermatology accepted article preview online, 20 January 2014.
nitric oxide, ultraviolet, skin, blood pressure, photolysis, nitrate, nitrite
0022-202X
1-38
Liu, Donald
a953db05-a652-4f67-a223-2c454f31250e
Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Hamilton, Alistair
d82f2e89-96fd-4030-87e2-0cbe9b93d56d
Lang, Ninian N.
38d83d94-a540-4053-b6ef-582d43149e44
Gallagher, Julie M.C.
dc8937ed-a0c5-4d73-ae5a-2d995ec52739
Newby, David E.
8684bf8c-1189-4f56-a666-c122bd9073c5
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Weller, Richard B
f78cfd69-d557-4fef-bd8d-28a90367feea
Liu, Donald
a953db05-a652-4f67-a223-2c454f31250e
Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Hamilton, Alistair
d82f2e89-96fd-4030-87e2-0cbe9b93d56d
Lang, Ninian N.
38d83d94-a540-4053-b6ef-582d43149e44
Gallagher, Julie M.C.
dc8937ed-a0c5-4d73-ae5a-2d995ec52739
Newby, David E.
8684bf8c-1189-4f56-a666-c122bd9073c5
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Weller, Richard B
f78cfd69-d557-4fef-bd8d-28a90367feea

Liu, Donald, Fernandez, Bernadette O., Hamilton, Alistair, Lang, Ninian N., Gallagher, Julie M.C., Newby, David E., Feelisch, Martin and Weller, Richard B (2014) UVA irradiation of human skin vasodilates arterial vasculature and lowers blood pressure independently of nitric oxide synthase. Journal of Investigative Dermatology, 1-38. (doi:10.1038/jid.2014.27). (PMID:24445737)

Record type: Article

Abstract

The incidence of hypertension and cardiovascular disease correlates with latitude and rises in winter. The molecular basis for this remains obscure. As nitric oxide (NO) metabolites are abundant in human skin we hypothesised that exposure to UVA may mobilise NO bioactivity into the circulation to exert beneficial cardiovascular effects independently of vitamin D. In 24 healthy volunteers irradiation of the skin with 2 Standard Erythemal Doses of UVA lowered BP, with concomitant decreases in circulating nitrate and rises in nitrite concentrations. Unexpectedly, acute dietary intervention aimed at modulating systemic nitrate availability had no effect on UV-induced hemodynamic changes, indicating that cardiovascular effects were not mediated via direct utilization of circulating nitrate. UVA irradiation of the forearm caused increased blood flow independently of NO-synthase activity, suggesting involvement of pre-formed cutaneous NO stores. Confocal fluorescence microscopy studies of human skin pre-labelled with the NO-imaging probe DAF2-DA revealed that UVA-induced NO release occurs in a NOS-independent, dose-dependent fashion, with the majority of the light-sensitive NO pool in the upper epidermis. Collectively, our data provide mechanistic insights into an important function of the skin in modulating systemic NO bioavailability which may account for the latitudinal and seasonal variations of BP and cardiovascular disease.Journal of Investigative Dermatology accepted article preview online, 20 January 2014.

Text
2014 Li - jid201427a with Suppl.pdf - Other
Download (2MB)

More information

e-pub ahead of print date: 20 January 2014
Keywords: nitric oxide, ultraviolet, skin, blood pressure, photolysis, nitrate, nitrite
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 361502
URI: http://eprints.soton.ac.uk/id/eprint/361502
ISSN: 0022-202X
PURE UUID: a517a942-95e6-4be6-9454-d7984d14dc82
ORCID for Bernadette O. Fernandez: ORCID iD orcid.org/0000-0001-6337-0381
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

Catalogue record

Date deposited: 23 Jan 2014 15:33
Last modified: 18 Feb 2021 17:20

Export record

Altmetrics

Contributors

Author: Donald Liu
Author: Bernadette O. Fernandez ORCID iD
Author: Alistair Hamilton
Author: Ninian N. Lang
Author: Julie M.C. Gallagher
Author: David E. Newby
Author: Martin Feelisch ORCID iD
Author: Richard B Weller

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×