Effect of oxygen tension on the amino acid utilisation of human embryonic stem cells
Effect of oxygen tension on the amino acid utilisation of human embryonic stem cells
Background/aims: human embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, a greater understanding of the nutrient requirements of hESCs will allow production of more appropriate culture media. This study aims to investigate the effect of environmental oxygen tension on the amino acid metabolism of hESCs.
Methods: the production or depletion of amino acids by hESCs cultured at 5% or 20% oxygen in the presence or absence of FGF2 was measured by reversephase HPLC.
Results: atmospheric oxygen, or removal of FGF2 from hESCs cultured at 5% oxygen, perturbed the uptake or release of individual amino acids and the total amino acid turnover compared to hESCs cultured at 5% oxygen. In particular, serine uptake was reduced at 20% oxygen and by removal of FGF2.
Conclusions: highly pluripotent hESCs, cultured at 5% oxygen, demonstrate a greater amino acid turnover than hESCs cultured at 20% oxygen, or without FGF2. These data suggest that amino acid turnover could be used as a measure of the self-renewal capacity of hESCs
embryonic stem cells, amino acids, hypoxia, oxygen tension, metabolism
237-246
Christensen, David R.
f8d74902-963b-433c-98ba-11e13700f2df
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Houghton, Franchesca D.
53946041-127e-45a8-9edb-bf4b3c23005f
February 2014
Christensen, David R.
f8d74902-963b-433c-98ba-11e13700f2df
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Houghton, Franchesca D.
53946041-127e-45a8-9edb-bf4b3c23005f
Christensen, David R., Calder, Philip C. and Houghton, Franchesca D.
(2014)
Effect of oxygen tension on the amino acid utilisation of human embryonic stem cells.
Cellular Physiology and Biochemistry, 33 (1), .
(doi:10.1159/000356665).
Abstract
Background/aims: human embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, a greater understanding of the nutrient requirements of hESCs will allow production of more appropriate culture media. This study aims to investigate the effect of environmental oxygen tension on the amino acid metabolism of hESCs.
Methods: the production or depletion of amino acids by hESCs cultured at 5% or 20% oxygen in the presence or absence of FGF2 was measured by reversephase HPLC.
Results: atmospheric oxygen, or removal of FGF2 from hESCs cultured at 5% oxygen, perturbed the uptake or release of individual amino acids and the total amino acid turnover compared to hESCs cultured at 5% oxygen. In particular, serine uptake was reduced at 20% oxygen and by removal of FGF2.
Conclusions: highly pluripotent hESCs, cultured at 5% oxygen, demonstrate a greater amino acid turnover than hESCs cultured at 20% oxygen, or without FGF2. These data suggest that amino acid turnover could be used as a measure of the self-renewal capacity of hESCs
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356665
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e-pub ahead of print date: 29 January 2014
Published date: February 2014
Keywords:
embryonic stem cells, amino acids, hypoxia, oxygen tension, metabolism
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 361938
URI: http://eprints.soton.ac.uk/id/eprint/361938
ISSN: 1015-8987
PURE UUID: 87325de8-bf98-4e91-96d5-415fbf4fad62
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Date deposited: 07 Feb 2014 14:56
Last modified: 15 Mar 2024 03:25
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Author:
David R. Christensen
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