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Controlled generation of microspheres incorporating extracellular matrix fibrils for three-dimensional cell culture

Controlled generation of microspheres incorporating extracellular matrix fibrils for three-dimensional cell culture
Controlled generation of microspheres incorporating extracellular matrix fibrils for three-dimensional cell culture
A growing body of evidence suggests that studying cell biology in classical two‐dimensional formats, such as cell culture plasticware, results in misleading, non‐physiological findings. This paper describes the optimization of a microsphere‐based system permitting 3D cell culture incorporating physiological extracellular matrix components. Bio‐electrospraying, the most advanced method currently available, is used to produce microspheres containing THP‐1 cells as a model cell line. The bio‐electrospraying para­meters of nozzle size, polymer flow rate, and voltage are systematically investigated in order to allow stable production of size‐controlled microspheres containing extracellular matrix material and human cells. The effect of bio‐electrospraying parameters, alginate type and cell concentration on cell viability are investigated using trypan blue and propidium iodide staining. Bio‐electrospraying has no effect on cell viability nor the ability of cells to proliferate. Cell viability is similarly minimally affected by encapsulation in all types of alginate tested (MVM, MVG, chemical and food‐grade). Cell density of 5 × 106 cells mL−1 within microspheres is the optimum for cell survival and proliferation. The stable generation of microspheres incorporating cells and extracellular matrix for use in a 3D cell culture will benefit study of many diverse diseases and permit investigation of cellular biology within a 3D matrix.
1616-301X
2648-2657
Workman, V.L.
d005e8ea-831d-4072-b28e-2716e31e9741
Tezera, L.B
c5598dbf-23a8-4934-96a4-7c783bf9e776
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Jayasinghe, S.N.
45ee9e92-565c-4485-829a-1c94f4837ba9
Workman, V.L.
d005e8ea-831d-4072-b28e-2716e31e9741
Tezera, L.B
c5598dbf-23a8-4934-96a4-7c783bf9e776
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Jayasinghe, S.N.
45ee9e92-565c-4485-829a-1c94f4837ba9

Workman, V.L., Tezera, L.B, Elkington, Paul T. and Jayasinghe, S.N. (2014) Controlled generation of microspheres incorporating extracellular matrix fibrils for three-dimensional cell culture. Advanced Functional Materials, 2648-2657. (doi:10.1002/adfm.201303891).

Record type: Article

Abstract

A growing body of evidence suggests that studying cell biology in classical two‐dimensional formats, such as cell culture plasticware, results in misleading, non‐physiological findings. This paper describes the optimization of a microsphere‐based system permitting 3D cell culture incorporating physiological extracellular matrix components. Bio‐electrospraying, the most advanced method currently available, is used to produce microspheres containing THP‐1 cells as a model cell line. The bio‐electrospraying para­meters of nozzle size, polymer flow rate, and voltage are systematically investigated in order to allow stable production of size‐controlled microspheres containing extracellular matrix material and human cells. The effect of bio‐electrospraying parameters, alginate type and cell concentration on cell viability are investigated using trypan blue and propidium iodide staining. Bio‐electrospraying has no effect on cell viability nor the ability of cells to proliferate. Cell viability is similarly minimally affected by encapsulation in all types of alginate tested (MVM, MVG, chemical and food‐grade). Cell density of 5 × 106 cells mL−1 within microspheres is the optimum for cell survival and proliferation. The stable generation of microspheres incorporating cells and extracellular matrix for use in a 3D cell culture will benefit study of many diverse diseases and permit investigation of cellular biology within a 3D matrix.

Full text not available from this repository.

More information

Accepted/In Press date: 9 December 2013
e-pub ahead of print date: 13 January 2014
Published date: 14 May 2014
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 361981
URI: https://eprints.soton.ac.uk/id/eprint/361981
ISSN: 1616-301X
PURE UUID: 7c753f94-e0cb-4d45-9be1-7bfb5a1a5f0f
ORCID for L.B Tezera: ORCID iD orcid.org/0000-0002-7898-6709
ORCID for Paul T. Elkington: ORCID iD orcid.org/0000-0003-0390-0613

Catalogue record

Date deposited: 10 Feb 2014 14:20
Last modified: 05 Nov 2019 01:39

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