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The ?-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana

The ?-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana
The ?-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana
Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G? subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated that the G? subunit may play a role in response to jasmonic acid (JA). Consistent with this, G? mutants showed a reduced response to JA in inhibition of chlorophyll accumulation and root growth, whilst G? gain-of-function plants overexpressing G? showed the opposite phenotype. The levels of JA and related compounds were unaffected in the gpa1-1 mutant, as was autoregulation of the Allene Oxide Synthase (AOS) gene that encodes a key enzyme for JA biosynthesis. In contrast, further analyses using G? loss- and gain-of-function Arabidopsis lines indicated that G? positively modulates the expression of the Vegetative Storage Protein (VSP) gene. This indicates that the G? subunit regulates a subset of JA-regulated genes defining a branch point in this signalling pathway in Arabidopsis. Further analysis of the impact of G? loss of function upon the JA-regulated transcriptome using Arabidopsis full genome arrays indicated that up to 29% of genes that are >2-fold regulated by JA in the wild type are misregulated in the G? mutant. This supports the observation that a significant proportion of, but not all, JA-regulated gene expression is mediated by G?
0022-0957
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Gobel, Cornelia
0b59b8e7-5d43-4103-b91d-94381804622a
Capper, Richard G.
6b2ba058-097c-4941-a323-e0e235af7286
Saunders, Nigel
42226608-aef6-442e-8845-7a7a701bfbfe
Feussner, Ivo
b365a90a-ae19-474c-ab3a-a21033b21813
Knight, Marc C.
add06d48-8822-4c27-89fb-5a59384bb4dd
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Gobel, Cornelia
0b59b8e7-5d43-4103-b91d-94381804622a
Capper, Richard G.
6b2ba058-097c-4941-a323-e0e235af7286
Saunders, Nigel
42226608-aef6-442e-8845-7a7a701bfbfe
Feussner, Ivo
b365a90a-ae19-474c-ab3a-a21033b21813
Knight, Marc C.
add06d48-8822-4c27-89fb-5a59384bb4dd

Okamoto, Haruko, Gobel, Cornelia, Capper, Richard G., Saunders, Nigel, Feussner, Ivo and Knight, Marc C. (2009) The ?-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana. Journal of Experimental Botany, 60 (7). (doi:10.1093/jxb/erp060).

Record type: Article

Abstract

Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G? subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated that the G? subunit may play a role in response to jasmonic acid (JA). Consistent with this, G? mutants showed a reduced response to JA in inhibition of chlorophyll accumulation and root growth, whilst G? gain-of-function plants overexpressing G? showed the opposite phenotype. The levels of JA and related compounds were unaffected in the gpa1-1 mutant, as was autoregulation of the Allene Oxide Synthase (AOS) gene that encodes a key enzyme for JA biosynthesis. In contrast, further analyses using G? loss- and gain-of-function Arabidopsis lines indicated that G? positively modulates the expression of the Vegetative Storage Protein (VSP) gene. This indicates that the G? subunit regulates a subset of JA-regulated genes defining a branch point in this signalling pathway in Arabidopsis. Further analysis of the impact of G? loss of function upon the JA-regulated transcriptome using Arabidopsis full genome arrays indicated that up to 29% of genes that are >2-fold regulated by JA in the wild type are misregulated in the G? mutant. This supports the observation that a significant proportion of, but not all, JA-regulated gene expression is mediated by G?

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Published date: 2009
Organisations: Centre for Biological Sciences

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Local EPrints ID: 362157
URI: http://eprints.soton.ac.uk/id/eprint/362157
ISSN: 0022-0957
PURE UUID: 934b2bae-e8e9-4dec-b977-090cd0edd86e

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Date deposited: 13 Mar 2014 09:30
Last modified: 14 Mar 2024 16:01

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Contributors

Author: Haruko Okamoto
Author: Cornelia Gobel
Author: Richard G. Capper
Author: Nigel Saunders
Author: Ivo Feussner
Author: Marc C. Knight

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