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Jasmonate and phytochrome A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability

Jasmonate and phytochrome A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability
Jasmonate and phytochrome A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability
Jasmonate (JA) activates plant defense, promotes pollen maturation, and suppresses plant growth. An emerging theme in JA biology is its involvement in light responses; here, we examine the interdependence of the JA- and light-signaling pathways in Arabidopsis thaliana. We demonstrate that mutants deficient in JA biosynthesis and signaling are deficient in a subset of high irradiance responses in far-red (FR) light. These mutants display exaggerated shade responses to low, but not high, R/FR ratio light, suggesting a role for JA in phytochrome A (phyA) signaling. Additionally, we demonstrate that the FR light-induced expression of transcription factor genes is dependent on CORONATINE INSENSITIVE1 (COI1), a central component of JA signaling, and is suppressed by JA. phyA mutants had reduced JA-regulated growth inhibition and VSP expression and increased content of cis-(+)-12-oxophytodienoic acid, an intermediate in JA biosynthesis. Significantly, COI1-mediated degradation of JASMONATE ZIM DOMAIN1-beta-glucuronidase (JAZ1-GUS) in response to mechanical wounding and JA treatment required phyA, and ectopic expression of JAZ1-GUS resulted in exaggerated shade responses. Together, these results indicate that JA and phyA signaling are integrated through degradation of the JAZ1 protein, and both are required for plant responses to light and stress
1040-4651
1143-1160
Robson, Francis
8bbcbfca-08c5-4222-818b-51ac01cb003a
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Patrick, Elaine
c6f2379a-5b16-4a35-87bc-8f4203a9cb80
Harris, Sue-Re
870b530c-c0a7-43cf-bc10-924384bd19d9
Wasternack, Claus
bb35f657-5956-4cd1-8770-b87bbfc58bea
Brearley, Charles
11871f90-4a53-4bcd-8b10-97d30e585744
Turner, John G.
68c3b16a-d61c-4daa-92e3-5aaaca84a99b
Robson, Francis
8bbcbfca-08c5-4222-818b-51ac01cb003a
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Patrick, Elaine
c6f2379a-5b16-4a35-87bc-8f4203a9cb80
Harris, Sue-Re
870b530c-c0a7-43cf-bc10-924384bd19d9
Wasternack, Claus
bb35f657-5956-4cd1-8770-b87bbfc58bea
Brearley, Charles
11871f90-4a53-4bcd-8b10-97d30e585744
Turner, John G.
68c3b16a-d61c-4daa-92e3-5aaaca84a99b

Robson, Francis, Okamoto, Haruko, Patrick, Elaine, Harris, Sue-Re, Wasternack, Claus, Brearley, Charles and Turner, John G. (2010) Jasmonate and phytochrome A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability. The Plant Cell, 22 (4), 1143-1160. (doi:10.1105/tpc.109.067728). (PMID:20435902)

Record type: Article

Abstract

Jasmonate (JA) activates plant defense, promotes pollen maturation, and suppresses plant growth. An emerging theme in JA biology is its involvement in light responses; here, we examine the interdependence of the JA- and light-signaling pathways in Arabidopsis thaliana. We demonstrate that mutants deficient in JA biosynthesis and signaling are deficient in a subset of high irradiance responses in far-red (FR) light. These mutants display exaggerated shade responses to low, but not high, R/FR ratio light, suggesting a role for JA in phytochrome A (phyA) signaling. Additionally, we demonstrate that the FR light-induced expression of transcription factor genes is dependent on CORONATINE INSENSITIVE1 (COI1), a central component of JA signaling, and is suppressed by JA. phyA mutants had reduced JA-regulated growth inhibition and VSP expression and increased content of cis-(+)-12-oxophytodienoic acid, an intermediate in JA biosynthesis. Significantly, COI1-mediated degradation of JASMONATE ZIM DOMAIN1-beta-glucuronidase (JAZ1-GUS) in response to mechanical wounding and JA treatment required phyA, and ectopic expression of JAZ1-GUS resulted in exaggerated shade responses. Together, these results indicate that JA and phyA signaling are integrated through degradation of the JAZ1 protein, and both are required for plant responses to light and stress

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Published date: 2010
Organisations: Centre for Biological Sciences

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Local EPrints ID: 362158
URI: http://eprints.soton.ac.uk/id/eprint/362158
ISSN: 1040-4651
PURE UUID: 77a94bb3-665e-478a-96ca-3b119a8951cd

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Date deposited: 14 Mar 2014 08:46
Last modified: 16 Jul 2019 21:12

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