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Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice

Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice
Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice
Bowman-Birk inhibitor (BBI) genes encode serine protease inhibitors that have repetitive cysteine-rich domains with reactive sites for the trypsin or chymotrypsin family. We have identified seven BBI genes from japonica rice (Oryza sativa subsp. japonica var Teqing). All of the genes identified were found in a single cluster on the southern end of the long arm of rice chromosome 1. Four of the seven BBI genes have two repetitive cysteine-rich domains, whereas one has a truncated domain with only one reactive site. We have also identified three novel BBI genes, each of which possesses three repetitive domains instead of two. In situ hybridization analyses indicated that the accumulation of rice BBI transcripts was differentially regulated in germinating embryos and also in the leaves, roots, and flower organs at later developmental stages. Different members of the rice BBI gene family displayed different expression patterns during rice seed germination, and wounding induced the expression of rice BBI transcripts. The three-domain BBIs had higher expression levels than the two-domain BBIs. It was also found that the mRNA of rice BBI genes was present in abundant amounts in scutellar epithelium and aleurone layer cells. RBBI3-1, one of the three-domain RBBI, exhibited in vitro trypsin-inhibiting activity but no chymotrypsin-inhibiting activity. Overexpression of RBBI2-3 in transgenic rice plants resulted in resistance to the fungal pathogen Pyricularia oryzae, indicating that proteinase inhibitors confer resistance against the fungal pathogen in vivo and that they might play a role in the defense system of the rice plant.
0032-0889
560-570
Qu, Li-Jia
33388626-6b99-4d2a-8a0d-d9b4f07842e2
Chen, Jun
ae8e5c6b-5ab6-4682-8ff4-519d7a2768a2
Liu, Meihua
cd272006-09ac-4ab8-9cbb-c659c9b9db3d
Pan, Naisui
156ef246-7f2a-4281-ab27-3b3c3c0c7e7e
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Lin, Zhongzhuan
1858fe1a-d078-406b-a15e-4da7632ffde4
Li, Chengyun
74e69363-3c45-4b00-aa45-8be8e4d3a5dd
Li, Donghui
0ff94eb7-4ae8-4b81-bae1-a6ee962cbdbf
Wang, Jinling
1eeb7d15-0ecc-422a-9b06-67bb2411bc20
Zhu, Guofeng
f5a1915c-7d77-4fa2-a6df-593825a5bd13
Zhao, Xin
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Chen, Xi
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Gu, Hongya
4b46c320-e4b0-4c16-b6aa-fa8d209f07f2
Chen, Zhangliang
e2be887f-304b-4957-aa24-0da798a5f98d
Qu, Li-Jia
33388626-6b99-4d2a-8a0d-d9b4f07842e2
Chen, Jun
ae8e5c6b-5ab6-4682-8ff4-519d7a2768a2
Liu, Meihua
cd272006-09ac-4ab8-9cbb-c659c9b9db3d
Pan, Naisui
156ef246-7f2a-4281-ab27-3b3c3c0c7e7e
Okamoto, Haruko
cea35380-7618-44c8-a268-47b0198cc7f9
Lin, Zhongzhuan
1858fe1a-d078-406b-a15e-4da7632ffde4
Li, Chengyun
74e69363-3c45-4b00-aa45-8be8e4d3a5dd
Li, Donghui
0ff94eb7-4ae8-4b81-bae1-a6ee962cbdbf
Wang, Jinling
1eeb7d15-0ecc-422a-9b06-67bb2411bc20
Zhu, Guofeng
f5a1915c-7d77-4fa2-a6df-593825a5bd13
Zhao, Xin
634e60cd-764f-48ab-9759-673fe78842d4
Chen, Xi
8bdb9873-52cb-4688-8cae-b4da945e0662
Gu, Hongya
4b46c320-e4b0-4c16-b6aa-fa8d209f07f2
Chen, Zhangliang
e2be887f-304b-4957-aa24-0da798a5f98d

Qu, Li-Jia, Chen, Jun, Liu, Meihua, Pan, Naisui, Okamoto, Haruko, Lin, Zhongzhuan, Li, Chengyun, Li, Donghui, Wang, Jinling, Zhu, Guofeng, Zhao, Xin, Chen, Xi, Gu, Hongya and Chen, Zhangliang (2003) Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice. Plant Physiology, 133 (2), 560-570. (doi:10.1104/pp.103.024810).

Record type: Article

Abstract

Bowman-Birk inhibitor (BBI) genes encode serine protease inhibitors that have repetitive cysteine-rich domains with reactive sites for the trypsin or chymotrypsin family. We have identified seven BBI genes from japonica rice (Oryza sativa subsp. japonica var Teqing). All of the genes identified were found in a single cluster on the southern end of the long arm of rice chromosome 1. Four of the seven BBI genes have two repetitive cysteine-rich domains, whereas one has a truncated domain with only one reactive site. We have also identified three novel BBI genes, each of which possesses three repetitive domains instead of two. In situ hybridization analyses indicated that the accumulation of rice BBI transcripts was differentially regulated in germinating embryos and also in the leaves, roots, and flower organs at later developmental stages. Different members of the rice BBI gene family displayed different expression patterns during rice seed germination, and wounding induced the expression of rice BBI transcripts. The three-domain BBIs had higher expression levels than the two-domain BBIs. It was also found that the mRNA of rice BBI genes was present in abundant amounts in scutellar epithelium and aleurone layer cells. RBBI3-1, one of the three-domain RBBI, exhibited in vitro trypsin-inhibiting activity but no chymotrypsin-inhibiting activity. Overexpression of RBBI2-3 in transgenic rice plants resulted in resistance to the fungal pathogen Pyricularia oryzae, indicating that proteinase inhibitors confer resistance against the fungal pathogen in vivo and that they might play a role in the defense system of the rice plant.

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e-pub ahead of print date: 28 August 2003
Published date: October 2003
Organisations: Centre for Biological Sciences

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Local EPrints ID: 362162
URI: http://eprints.soton.ac.uk/id/eprint/362162
ISSN: 0032-0889
PURE UUID: c8bf42a3-8146-49aa-a5c6-5b14b87be064

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Date deposited: 23 Apr 2014 13:53
Last modified: 16 Jul 2019 21:12

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Contributors

Author: Li-Jia Qu
Author: Jun Chen
Author: Meihua Liu
Author: Naisui Pan
Author: Haruko Okamoto
Author: Zhongzhuan Lin
Author: Chengyun Li
Author: Donghui Li
Author: Jinling Wang
Author: Guofeng Zhu
Author: Xin Zhao
Author: Xi Chen
Author: Hongya Gu
Author: Zhangliang Chen

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