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Control of mitosis by phytochrome and a blue-light receptor in fern spores

Control of mitosis by phytochrome and a blue-light receptor in fern spores
Control of mitosis by phytochrome and a blue-light receptor in fern spores
The first mitosis in spores of the fern A. capillus-veneris was observed under a microscope equipped with Nomarski optics with irradiation from a safelight at 900 nm, and under a fluorescent microscope after staining with 4[prime],6-diamidino-2-phenylindole. During imbibition the nucleus remained near one corner of each tetrahedron-shaped dormant spore, and asymmetric cell division occurred upon brief irradiation with red light. This red light-induced mitosis was photoreversibly prevented by subsequent brief exposure to far-red light and was photo-irreversibly prevented by brief irradiation with blue light. However, neither far-red nor blue light affected the germination rate when spores were irradiated after the first mitosis. Therefore, the first mitosis in the spores appears to be the crucial step for photoinduction of spore germination. Furthermore, experiments using a microbeam of red or blue light demonstrated that blue light was effective only when exposed to the nucleus, and no specific intracellular photoreceptive site for red light was found in the spores. Therefore, phytochrome in the far-red absorbing form induces the first mitosis in germinating spores but prevents the subsequent mitosis in protonemata, whereas a blue-light receptor prevents the former but induces the latter
0032-0889
677-683
Furuya, M.
d9dc9903-d1c8-4a71-a22a-01a6c8ea8813
Kanno, M.
d619b5cc-daa3-4dbc-b3c0-b0bfa2d490e6
Okamoto, H.
cea35380-7618-44c8-a268-47b0198cc7f9
Fukada, S.
1f92dc8a-6e2d-4af0-ab7b-ff080d42cfef
Wada, M.
41c99f27-384c-4952-b7dd-8a1c1f554c16
Furuya, M.
d9dc9903-d1c8-4a71-a22a-01a6c8ea8813
Kanno, M.
d619b5cc-daa3-4dbc-b3c0-b0bfa2d490e6
Okamoto, H.
cea35380-7618-44c8-a268-47b0198cc7f9
Fukada, S.
1f92dc8a-6e2d-4af0-ab7b-ff080d42cfef
Wada, M.
41c99f27-384c-4952-b7dd-8a1c1f554c16

Furuya, M., Kanno, M., Okamoto, H., Fukada, S. and Wada, M. (1997) Control of mitosis by phytochrome and a blue-light receptor in fern spores. Plant Physiology, 113 (3), 677-683. (doi:10.1104/pp.113.3.677). (PMID:12223635)

Record type: Article

Abstract

The first mitosis in spores of the fern A. capillus-veneris was observed under a microscope equipped with Nomarski optics with irradiation from a safelight at 900 nm, and under a fluorescent microscope after staining with 4[prime],6-diamidino-2-phenylindole. During imbibition the nucleus remained near one corner of each tetrahedron-shaped dormant spore, and asymmetric cell division occurred upon brief irradiation with red light. This red light-induced mitosis was photoreversibly prevented by subsequent brief exposure to far-red light and was photo-irreversibly prevented by brief irradiation with blue light. However, neither far-red nor blue light affected the germination rate when spores were irradiated after the first mitosis. Therefore, the first mitosis in the spores appears to be the crucial step for photoinduction of spore germination. Furthermore, experiments using a microbeam of red or blue light demonstrated that blue light was effective only when exposed to the nucleus, and no specific intracellular photoreceptive site for red light was found in the spores. Therefore, phytochrome in the far-red absorbing form induces the first mitosis in germinating spores but prevents the subsequent mitosis in protonemata, whereas a blue-light receptor prevents the former but induces the latter

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Published date: 1997
Organisations: Centre for Biological Sciences

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Local EPrints ID: 362181
URI: http://eprints.soton.ac.uk/id/eprint/362181
ISSN: 0032-0889
PURE UUID: 9c1fcf5c-d74c-4dbd-ad6e-f9479f0c9cbd

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Date deposited: 14 Mar 2014 10:24
Last modified: 14 Mar 2024 16:01

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Contributors

Author: M. Furuya
Author: M. Kanno
Author: H. Okamoto
Author: S. Fukada
Author: M. Wada

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