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Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome A

Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome A
Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome A
Phytochrome (phy) in etiolated seedlings of wild-type (WT) Arabidopsis (Ler) and its transgenic lines (TL) L15 and L20 transformed with Adiantum capillus-veneris PHY1 cDNA (Okamoto et al., 1997) was investigated using low-temperature (85 K) fluorescence spectroscopy and photochemistry. It was found that while WT seed germination requires stimulation by light, the TL germinated equally well with or without pre-illumination. Phytochrome content [Ptot] was 2-fold higher in TL whereas the level of Pr ? lumi-R phototransformation at 85 K (?1) was similar between WT (0.25) and TL (0.27). When seeds germinated with pre-illumination, the proportion of the photochemical types Pr? active and Pr? inactive at 85 K was 50/50 in WT and 54/46 in TL, respectively. Dark-germinated TL had a ?1 value of 0.16 and the proportion of Pr? and Pr? was 32/68, respectively, without changes in [Ptot]. Evaluations based on these data revealed that phy1 has Pr? and Pr?, designated phy1? and phy1?, akin to phyA, which comprises both Pr photochemical types (phyA? and phyA?), and in contrast to phyB that possesses only Pr?. The proportion of phy1? and phy1? depends on pre-illumination for induction of germination. The pigment most likely accumulated in the seeds and was active in promoting Arabidopsis seed germination
1011-1344
20-29
Sineshchekov, V.
c81511ab-9de6-40ca-9df1-ce204b5cc2f2
Koppel, L.
3d88f2bf-bc02-4e51-b895-88bc8bd9e152
Okamoto, H.
cea35380-7618-44c8-a268-47b0198cc7f9
Wada, M.
41c99f27-384c-4952-b7dd-8a1c1f554c16
Sineshchekov, V.
c81511ab-9de6-40ca-9df1-ce204b5cc2f2
Koppel, L.
3d88f2bf-bc02-4e51-b895-88bc8bd9e152
Okamoto, H.
cea35380-7618-44c8-a268-47b0198cc7f9
Wada, M.
41c99f27-384c-4952-b7dd-8a1c1f554c16

Sineshchekov, V., Koppel, L., Okamoto, H. and Wada, M. (2014) Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome A. Journal of Photochemistry and Photobiology B: Biology, 130, 20-29. (doi:10.1016/j.jphotobiol.2013.10.007). (PMID:24246712)

Record type: Article

Abstract

Phytochrome (phy) in etiolated seedlings of wild-type (WT) Arabidopsis (Ler) and its transgenic lines (TL) L15 and L20 transformed with Adiantum capillus-veneris PHY1 cDNA (Okamoto et al., 1997) was investigated using low-temperature (85 K) fluorescence spectroscopy and photochemistry. It was found that while WT seed germination requires stimulation by light, the TL germinated equally well with or without pre-illumination. Phytochrome content [Ptot] was 2-fold higher in TL whereas the level of Pr ? lumi-R phototransformation at 85 K (?1) was similar between WT (0.25) and TL (0.27). When seeds germinated with pre-illumination, the proportion of the photochemical types Pr? active and Pr? inactive at 85 K was 50/50 in WT and 54/46 in TL, respectively. Dark-germinated TL had a ?1 value of 0.16 and the proportion of Pr? and Pr? was 32/68, respectively, without changes in [Ptot]. Evaluations based on these data revealed that phy1 has Pr? and Pr?, designated phy1? and phy1?, akin to phyA, which comprises both Pr photochemical types (phyA? and phyA?), and in contrast to phyB that possesses only Pr?. The proportion of phy1? and phy1? depends on pre-illumination for induction of germination. The pigment most likely accumulated in the seeds and was active in promoting Arabidopsis seed germination

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Published date: 2014
Organisations: Centre for Biological Sciences

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Local EPrints ID: 362182
URI: http://eprints.soton.ac.uk/id/eprint/362182
ISSN: 1011-1344
PURE UUID: a1d26bbf-a78a-44bf-b16e-401b2c321628

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Date deposited: 14 Mar 2014 11:36
Last modified: 16 Jul 2019 21:12

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