Rotational catalysis in proton pumping ATPases: From E. coli F-ATPase to mammalian V-ATPase

Futai, Masamitsu, Nakanishi-Matsui, Mayumi, Okamoto, Haruko, Sekiya, Mizuki and Nakamoto, Robert K. (2012) Rotational catalysis in proton pumping ATPases: From E. coli F-ATPase to mammalian V-ATPase. [in special issue: 17th European Bioenergetics Conference] Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1817 (10), 1711-1721. (doi:10.1016/j.bbabio.2012.03.015). (PMID:22459334)

Abstract

We focus on the rotational catalysis of Escherichia coli F-ATPase (ATP synthase, FOF1). Using a probe with low viscous drag, we found stochastic fluctuation of the rotation rates, a flat energy pathway, and contribution of an inhibited state to the overall behavior of the enzyme. Mutational analyses revealed the importance of the interactions among ? and ? subunits and the ? subunit catalytic domain. We also discuss the V-ATPase, which has different physiological roles from the F-ATPase, but is structurally and mechanistically similar. We review the rotation, diversity of subunits, and the regulatory mechanism of reversible subunit dissociation/assembly of Saccharomyces cerevisiae and mammalian complexes

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