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CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help

CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help
CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help
CD40 is essential in enabling antigen-presenting cells to process and present antigen effectively to T cells. We demonstrate here that when antibody against CD40 is used to treat mice with syngeneic lymphoma, a rapid cytotoxic T-cell response independent of T-helper cells occurs, with tenfold expansion of CD8 + T cells over a period of 5 days. This response eradicates the lymphoma and provides protection against tumor rechallenge without further antibody treatment. Thus, it seems that by treating mice with monoclonal antibody against CD40, we are immunizing against syngeneic tumors. The phenomenon proved reproducible with two antibodies against CD40 (3/23 and FGK-45) in three CD40+ lymphomas (A20, A31 and BCL1) and gave partial protection in one of two CD40? lymphomas (EL4 and Ten1). Although the nature of the target antigens on these lymphomas is unknown, CD8+ T cells recovered from responding mice showed powerful cytotoxic activity against the target B-cell lymphoma in vitro.
1078-8956
549-553
French, R.R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Chan, H.T.C.
b109c93f-7e9a-44ee-ad12-da757b1b11fc
Tutt, A.L.
46ce577b-aea1-412d-84ea-fc4dab794469
Glennie, M.J.
9f6f0eff-4560-48c2-80cd-0ec116110ded
French, R.R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Chan, H.T.C.
b109c93f-7e9a-44ee-ad12-da757b1b11fc
Tutt, A.L.
46ce577b-aea1-412d-84ea-fc4dab794469
Glennie, M.J.
9f6f0eff-4560-48c2-80cd-0ec116110ded

French, R.R., Chan, H.T.C., Tutt, A.L. and Glennie, M.J. (1999) CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help. Nature Medicine, 5 (5), 549-553. (doi:10.1038/8426). (PMID:10229232)

Record type: Article

Abstract

CD40 is essential in enabling antigen-presenting cells to process and present antigen effectively to T cells. We demonstrate here that when antibody against CD40 is used to treat mice with syngeneic lymphoma, a rapid cytotoxic T-cell response independent of T-helper cells occurs, with tenfold expansion of CD8 + T cells over a period of 5 days. This response eradicates the lymphoma and provides protection against tumor rechallenge without further antibody treatment. Thus, it seems that by treating mice with monoclonal antibody against CD40, we are immunizing against syngeneic tumors. The phenomenon proved reproducible with two antibodies against CD40 (3/23 and FGK-45) in three CD40+ lymphomas (A20, A31 and BCL1) and gave partial protection in one of two CD40? lymphomas (EL4 and Ten1). Although the nature of the target antigens on these lymphomas is unknown, CD8+ T cells recovered from responding mice showed powerful cytotoxic activity against the target B-cell lymphoma in vitro.

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Published date: May 1999
Organisations: Cancer Sciences

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Local EPrints ID: 362518
URI: http://eprints.soton.ac.uk/id/eprint/362518
ISSN: 1078-8956
PURE UUID: 0c0e7f24-db0a-4cf7-8a5f-fc643bd4178e

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Date deposited: 26 Feb 2014 12:44
Last modified: 16 Jul 2019 21:11

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Contributors

Author: R.R. French
Author: H.T.C. Chan
Author: A.L. Tutt
Author: M.J. Glennie

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