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Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways.

Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways.
Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways.
We have examined the mucosal changes occurring in bronchial biopsies from six atopic asthmatics 5-6 h after local endobronchial allergen challenge and compared them with biopsies from saline-challenged segments from the same subjects at the same time point. All the subjects developed localized bronchoconstriction in the allergen-challenged segment and had a decrease in forced expiratory volume in 1 s (FEV1) (P < 0.01) and a decrease in their methacholine provocative concentration of agonist required to reduce FEV1 from baseline by 20% (P < 0.05) 24 h postchallenge. At 6 h we observed an increase in neutrophils (P = 0.03), eosinophils (P = 0.025), mast cells (P = 0.03), and CD3+ lymphocytes (P = 0.025), but not in CD4+ or CD8+ lymphocyte counts. We also detected an increase in endothelial intercellular adhesion molecule type 1 (P < 0.05) and E-selectin (P < 0.005), but not vascular cell adhesion molecule type 1 expression with a correlative increase in submucosal and epithelial LFA+ leucocytes (P < 0.01). Thus, in sensitized asthmatics, local endobronchial allergen instillation leads to an increased inflammatory cell infiltrate of the airway mucosa that involves upregulation of specific adhesion molecules expressed on the microvasculature.
0021-9738
1411-1421
Montefort, S.
27326fc5-3106-4318-ad2b-39050fd0f3e2
Gratziou, C.
b686baec-20ee-419d-9f15-1bacdb6e2e0a
Goulding, D.
c815b264-ddfa-430f-b0f2-6b4204ecf491
Polosa, R.
34c9ef10-2fea-44a4-9521-63098d4c2571
Haskard, D.O.
5608542d-7c46-4d41-add9-d87436afc1b5
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Carroll, M.P.
f524e9ee-6db4-4e3f-be7a-69c97d7af0a5
Montefort, S.
27326fc5-3106-4318-ad2b-39050fd0f3e2
Gratziou, C.
b686baec-20ee-419d-9f15-1bacdb6e2e0a
Goulding, D.
c815b264-ddfa-430f-b0f2-6b4204ecf491
Polosa, R.
34c9ef10-2fea-44a4-9521-63098d4c2571
Haskard, D.O.
5608542d-7c46-4d41-add9-d87436afc1b5
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Carroll, M.P.
f524e9ee-6db4-4e3f-be7a-69c97d7af0a5

Montefort, S., Gratziou, C., Goulding, D., Polosa, R., Haskard, D.O., Howarth, P.H., Holgate, S.T. and Carroll, M.P. (1994) Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways. Journal of Clinical Investigation, 93 (4), 1411-1421. (PMID:7512980)

Record type: Article

Abstract

We have examined the mucosal changes occurring in bronchial biopsies from six atopic asthmatics 5-6 h after local endobronchial allergen challenge and compared them with biopsies from saline-challenged segments from the same subjects at the same time point. All the subjects developed localized bronchoconstriction in the allergen-challenged segment and had a decrease in forced expiratory volume in 1 s (FEV1) (P < 0.01) and a decrease in their methacholine provocative concentration of agonist required to reduce FEV1 from baseline by 20% (P < 0.05) 24 h postchallenge. At 6 h we observed an increase in neutrophils (P = 0.03), eosinophils (P = 0.025), mast cells (P = 0.03), and CD3+ lymphocytes (P = 0.025), but not in CD4+ or CD8+ lymphocyte counts. We also detected an increase in endothelial intercellular adhesion molecule type 1 (P < 0.05) and E-selectin (P < 0.005), but not vascular cell adhesion molecule type 1 expression with a correlative increase in submucosal and epithelial LFA+ leucocytes (P < 0.01). Thus, in sensitized asthmatics, local endobronchial allergen instillation leads to an increased inflammatory cell infiltrate of the airway mucosa that involves upregulation of specific adhesion molecules expressed on the microvasculature.

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Published date: April 1994
Organisations: Clinical & Experimental Sciences

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Local EPrints ID: 362519
URI: https://eprints.soton.ac.uk/id/eprint/362519
ISSN: 0021-9738
PURE UUID: 8a8abeb3-702b-4c67-a430-98cb395c5025

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Date deposited: 26 Feb 2014 12:46
Last modified: 08 Nov 2017 04:50

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