The University of Southampton
University of Southampton Institutional Repository

Dendritic cells in normal and asthmatic airways: expression of the ? subunit of the high affinity immunoglobulin E receptor (Fc?RI-?)

Dendritic cells in normal and asthmatic airways: expression of the ? subunit of the high affinity immunoglobulin E receptor (Fc?RI-?)
Dendritic cells in normal and asthmatic airways: expression of the ? subunit of the high affinity immunoglobulin E receptor (Fc?RI-?)
BACKGROUND: Immunoglobulin E (IgE) plays an important role in asthma, with total serum IgE levels closely related to both clinical expression of the disease and airway hyperresponsiveness. IgE binds to a high affinity cell-surface receptor (Fc epsilon RI) which is present on mast cells and which has also recently been demonstrated on cutaneous dendritic cells. If pulmonary dendritic cells were also able to express this receptor, this would have important implications with regard to their potential role in asthma.

OBJECTIVES: The aims of the study were to investigate the expression of the alpha subunit of the high affinity IgE receptor (Fc epsilon RI-alpha) in normal and asthmatic airways, and to analyse its cellular provenance with particular emphasis on the dendritic cell.

METHODS: Bronchial biopsy specimens were obtained using fibreoptic bronchoscopy from 10 atopic asthmatics and nine non-atopic non-asthmatic control subjects. Specimens were processed into glycolmethacrylate resin and analysed by immunohistochemistry using specific monoclonal antibodies against Fc epsilon RI-alpha, and against tryptase and CD1a, markers for mast cells and dendritic cells, respectively.

RESULTS: The numbers of dendritic cells were significantly higher in the airways of asthmatics compared with those of control subjects (P < 0.02). Analysis of sequential sections revealed that the alpha subunit of Fc epsilon RI was localized to both mast cells and dendritic cells. The proportion of dendritic cells expressing Fc epsilon RI-alpha was significantly increased in the asthmatic group (P < 0.003).

CONCLUSION: These results support the hypothesis that dendritic cells play an important role in allergic asthma although the functional significance of Fc epsilon RI-alpha expression needs further investigation.
asthma, biopsy, bronchoscopy, dendritic cells, Fc?RI, immunoglobulin ?
0954-7894
648-655
Tunon-de-Lara, J.M.
49caf870-d8d1-486f-99ae-51fedd2622fd
Redington, A.E.
ecaab7c4-c44f-4f52-899a-18993a796c2e
Bradding, P.
39990c99-3c21-44b6-b1f7-084eddf29722
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Hartley, J.A.
73be7079-482a-4843-8068-09918db3cfee
Semper, A.E.
30f48e4a-8941-4707-b367-92bc9bc2b2af
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Tunon-de-Lara, J.M.
49caf870-d8d1-486f-99ae-51fedd2622fd
Redington, A.E.
ecaab7c4-c44f-4f52-899a-18993a796c2e
Bradding, P.
39990c99-3c21-44b6-b1f7-084eddf29722
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Hartley, J.A.
73be7079-482a-4843-8068-09918db3cfee
Semper, A.E.
30f48e4a-8941-4707-b367-92bc9bc2b2af
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc

Tunon-de-Lara, J.M., Redington, A.E., Bradding, P., Church, M.K., Hartley, J.A., Semper, A.E. and Holgate, S.T. (1996) Dendritic cells in normal and asthmatic airways: expression of the ? subunit of the high affinity immunoglobulin E receptor (Fc?RI-?). Clinical & Experimental Allergy, 26 (6), 648-655. (doi:10.1111/j.1365-2222.1996.tb00591.x). (PMID:8809422)

Record type: Article

Abstract

BACKGROUND: Immunoglobulin E (IgE) plays an important role in asthma, with total serum IgE levels closely related to both clinical expression of the disease and airway hyperresponsiveness. IgE binds to a high affinity cell-surface receptor (Fc epsilon RI) which is present on mast cells and which has also recently been demonstrated on cutaneous dendritic cells. If pulmonary dendritic cells were also able to express this receptor, this would have important implications with regard to their potential role in asthma.

OBJECTIVES: The aims of the study were to investigate the expression of the alpha subunit of the high affinity IgE receptor (Fc epsilon RI-alpha) in normal and asthmatic airways, and to analyse its cellular provenance with particular emphasis on the dendritic cell.

METHODS: Bronchial biopsy specimens were obtained using fibreoptic bronchoscopy from 10 atopic asthmatics and nine non-atopic non-asthmatic control subjects. Specimens were processed into glycolmethacrylate resin and analysed by immunohistochemistry using specific monoclonal antibodies against Fc epsilon RI-alpha, and against tryptase and CD1a, markers for mast cells and dendritic cells, respectively.

RESULTS: The numbers of dendritic cells were significantly higher in the airways of asthmatics compared with those of control subjects (P < 0.02). Analysis of sequential sections revealed that the alpha subunit of Fc epsilon RI was localized to both mast cells and dendritic cells. The proportion of dendritic cells expressing Fc epsilon RI-alpha was significantly increased in the asthmatic group (P < 0.003).

CONCLUSION: These results support the hypothesis that dendritic cells play an important role in allergic asthma although the functional significance of Fc epsilon RI-alpha expression needs further investigation.

Full text not available from this repository.

More information

Published date: June 1996
Keywords: asthma, biopsy, bronchoscopy, dendritic cells, Fc?RI, immunoglobulin ?
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 362524
URI: https://eprints.soton.ac.uk/id/eprint/362524
ISSN: 0954-7894
PURE UUID: 8b74e3f2-86ed-41cc-83ef-0caabca996fe

Catalogue record

Date deposited: 26 Feb 2014 12:49
Last modified: 18 Jul 2017 02:51

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×