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Gold nanoparticles explore cells: cellular uptake and their use as intracellular probes

Gold nanoparticles explore cells: cellular uptake and their use as intracellular probes
Gold nanoparticles explore cells: cellular uptake and their use as intracellular probes
Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.
gold nanoparticles, nanoparticle uptake, intracellular, imaging, surface-enhanced raman spectroscopy, SERS
1046-2023
1-20
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Septiadi, Dedy
1c5650ec-4942-4232-8503-468115afa973
Wilts, Bodo D.
3519769b-4e97-42c1-a69d-ba4a585d5435
Patel, Imran I.
1217bf39-9ae3-4e4c-8d83-06dfcf2ec523
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Fragniere, Alexandra
018be11f-5129-41fc-abb7-085cc119554a
Barker, Roger A.
64e07f34-66db-42a7-9a28-2cb3070f63ad
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Septiadi, Dedy
1c5650ec-4942-4232-8503-468115afa973
Wilts, Bodo D.
3519769b-4e97-42c1-a69d-ba4a585d5435
Patel, Imran I.
1217bf39-9ae3-4e4c-8d83-06dfcf2ec523
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Fragniere, Alexandra
018be11f-5129-41fc-abb7-085cc119554a
Barker, Roger A.
64e07f34-66db-42a7-9a28-2cb3070f63ad
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9

Huefner, Anna, Septiadi, Dedy, Wilts, Bodo D., Patel, Imran I., Kuan, Wei-Li, Fragniere, Alexandra, Barker, Roger A. and Mahajan, Sumeet (2014) Gold nanoparticles explore cells: cellular uptake and their use as intracellular probes. Methods, 1-20. (doi:10.1016/j.ymeth.2014.02.006). (PMID:24583117)

Record type: Article

Abstract

Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.

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Methods 2014 accepted manuscript.pdf - Author's Original
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e-pub ahead of print date: 28 February 2014
Published date: July 2014
Keywords: gold nanoparticles, nanoparticle uptake, intracellular, imaging, surface-enhanced raman spectroscopy, SERS
Organisations: University of Southampton, Institute for Life Sciences

Identifiers

Local EPrints ID: 362667
URI: http://eprints.soton.ac.uk/id/eprint/362667
ISSN: 1046-2023
PURE UUID: cb0c76eb-2106-4841-a737-5ccf867f4b82
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

Catalogue record

Date deposited: 04 Mar 2014 14:38
Last modified: 03 Dec 2019 01:45

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Contributors

Author: Anna Huefner
Author: Dedy Septiadi
Author: Bodo D. Wilts
Author: Imran I. Patel
Author: Wei-Li Kuan
Author: Alexandra Fragniere
Author: Roger A. Barker
Author: Sumeet Mahajan ORCID iD

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