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The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios

The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 ?atm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 ?atm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.
1664-302X
11p
Rouco, Mónica
f9af6260-7705-410c-aa52-a0ab10eec6e3
Branson, Oscar
ebe1d441-a235-463c-acbc-6048c6efefb8
Lebrato, Mario
0c14d20e-c641-4a61-8ba2-b6377cdf1777
Iglesias-Rodríguez, M. Débora
96ef46e8-5885-4124-a28d-53d1c1519dc5
Rouco, Mónica
f9af6260-7705-410c-aa52-a0ab10eec6e3
Branson, Oscar
ebe1d441-a235-463c-acbc-6048c6efefb8
Lebrato, Mario
0c14d20e-c641-4a61-8ba2-b6377cdf1777
Iglesias-Rodríguez, M. Débora
96ef46e8-5885-4124-a28d-53d1c1519dc5

Rouco, Mónica, Branson, Oscar, Lebrato, Mario and Iglesias-Rodríguez, M. Débora (2013) The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios. Frontiers in Microbiology, 4, 11p. (doi:10.3389/fmicb.2013.00155).

Record type: Article

Abstract

Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 ?atm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 ?atm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.

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More information

Published date: 18 June 2013
Organisations: Ocean and Earth Science

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Local EPrints ID: 362779
URI: http://eprints.soton.ac.uk/id/eprint/362779
ISSN: 1664-302X
PURE UUID: d4b0fd80-9d21-443f-b383-637024f96bae

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Date deposited: 06 Mar 2014 10:48
Last modified: 09 Jan 2022 09:46

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Contributors

Author: Mónica Rouco
Author: Oscar Branson
Author: Mario Lebrato
Author: M. Débora Iglesias-Rodríguez

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