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Diversity of the Luciferin binding protein gene in bioluminescent dinoflagellates – insights from a new gene in Noctiluca scintillans and sequences from gonyaulacoid genera

Diversity of the Luciferin binding protein gene in bioluminescent dinoflagellates – insights from a new gene in Noctiluca scintillans and sequences from gonyaulacoid genera
Diversity of the Luciferin binding protein gene in bioluminescent dinoflagellates – insights from a new gene in Noctiluca scintillans and sequences from gonyaulacoid genera
Dinoflagellate bioluminescence systems operate with or without a luciferin binding protein, representing two distinct modes of light production. However, the distribution, diversity, and evolution of the luciferin binding protein gene within bioluminescent dinoflagellates are not well known. We used PCR to detect and partially sequence this gene from the heterotrophic dinoflagellate Noctiluca scintillans and a group of ecologically important gonyaulacoid species. We report an additional luciferin binding protein gene in N. scintillans which is not attached to luciferase, further to its typical combined bioluminescence gene. This supports the hypothesis that a profound re-organization of the bioluminescence system has taken place in this organism. We also show that the luciferin binding protein gene is present in the genera Ceratocorys, Gonyaulax, and Protoceratium, and is prevalent in bioluminescent species of Alexandrium. Therefore, this gene is an integral component of the standard molecular bioluminescence machinery in dinoflagellates. Nucleotide sequences showed high within-strain variation among gene copies, revealing a highly diverse gene family comprising multiple gene types in some organisms. Phylogenetic analyses showed that, in some species, the evolution of the luciferin binding protein gene was different from the organism's general phylogenies, highlighting the complex evolutionary history of dinoflagellate bioluminescence systems.
1066-5234
134-145
Valiadi, Martha
e245e9c5-3e97-4896-b910-c4178c5fb3c9
Iglesias-Rodriguez, M.D.
f93f3be3-83b0-46c1-a5f8-e42ad1e30674
Valiadi, Martha
e245e9c5-3e97-4896-b910-c4178c5fb3c9
Iglesias-Rodriguez, M.D.
f93f3be3-83b0-46c1-a5f8-e42ad1e30674

Valiadi, Martha and Iglesias-Rodriguez, M.D. (2014) Diversity of the Luciferin binding protein gene in bioluminescent dinoflagellates – insights from a new gene in Noctiluca scintillans and sequences from gonyaulacoid genera. Journal of Eukaryotic Microbiology, 61 (2), 134-145. (doi:10.1111/jeu.12091).

Record type: Article

Abstract

Dinoflagellate bioluminescence systems operate with or without a luciferin binding protein, representing two distinct modes of light production. However, the distribution, diversity, and evolution of the luciferin binding protein gene within bioluminescent dinoflagellates are not well known. We used PCR to detect and partially sequence this gene from the heterotrophic dinoflagellate Noctiluca scintillans and a group of ecologically important gonyaulacoid species. We report an additional luciferin binding protein gene in N. scintillans which is not attached to luciferase, further to its typical combined bioluminescence gene. This supports the hypothesis that a profound re-organization of the bioluminescence system has taken place in this organism. We also show that the luciferin binding protein gene is present in the genera Ceratocorys, Gonyaulax, and Protoceratium, and is prevalent in bioluminescent species of Alexandrium. Therefore, this gene is an integral component of the standard molecular bioluminescence machinery in dinoflagellates. Nucleotide sequences showed high within-strain variation among gene copies, revealing a highly diverse gene family comprising multiple gene types in some organisms. Phylogenetic analyses showed that, in some species, the evolution of the luciferin binding protein gene was different from the organism's general phylogenies, highlighting the complex evolutionary history of dinoflagellate bioluminescence systems.

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Published date: March 2014
Organisations: Ocean and Earth Science

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Local EPrints ID: 363247
URI: http://eprints.soton.ac.uk/id/eprint/363247
ISSN: 1066-5234
PURE UUID: ae68cc26-0201-46f1-bdeb-8d5d1cd093fb

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Date deposited: 19 Mar 2014 11:39
Last modified: 21 Oct 2019 19:22

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