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Presoaking with BSS used for thin manually dissected DSEK (TMDSEK): a viable option for thin DSEK

Presoaking with BSS used for thin manually dissected DSEK (TMDSEK): a viable option for thin DSEK
Presoaking with BSS used for thin manually dissected DSEK (TMDSEK): a viable option for thin DSEK
Aim To describe a novel technique for the safe manual dissection of thin donor lenticules in 10 consecutive patients undergoing DSEK surgery.MethodsA key element of our new technique was to presoak the donor cornea in balanced salt solution (BSS) for 30?min before manual dissection. The cornea was placed on an artificial anterior chamber and pressure in the chamber was maintained at 80?mm?Hg. The limbus of the donor cornea was incised to the same depth as the central corneal thickness. Lamellar dissection was started with the short side of the Morlet dissector (Duckworth & Kent Ltd) and completed using the lamellar (less sharp) end of the Morlet dissector. Outcomes of 10 consecutive cases of thin manually dissected DSEK (TMDSEK) were prospectively analysed for thickness and visual outcome.ResultsMean graft thicknesses measured less than 100??m at 1 month post surgery (mean thickness 90.7??m, range 48-137??m, SD 29.96??m). Presoaked donor corneal pachymetry was strongly negatively correlated with graft thickness (correlation r=-0.75, P<0.05).DiscussionOur dissection technique achieves consistently thin endothelial donor corneal grafts that can be safely produced with minimal financial investment and no limitations on surgical time. This technique is likely to be of significant importance for a large proportion of the eye centres where microkeratomes may not be routinely available
0950-222X
Tsatsos, M.
82e13b1e-df1a-4351-8283-b4a0758b68cf
Konstantopoulos, Aris
7758cee7-c139-4ce0-96d3-ac4d3f4c1563
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Anderson, D.
441a2220-b721-417d-b9f4-8f40ed17a621
Tsatsos, M.
82e13b1e-df1a-4351-8283-b4a0758b68cf
Konstantopoulos, Aris
7758cee7-c139-4ce0-96d3-ac4d3f4c1563
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Anderson, D.
441a2220-b721-417d-b9f4-8f40ed17a621

Tsatsos, M., Konstantopoulos, Aris, Hossain, Parwez and Anderson, D. (2014) Presoaking with BSS used for thin manually dissected DSEK (TMDSEK): a viable option for thin DSEK. Eye. (doi:10.1038/eye.2014.36). (PMID:24625383)

Record type: Article

Abstract

Aim To describe a novel technique for the safe manual dissection of thin donor lenticules in 10 consecutive patients undergoing DSEK surgery.MethodsA key element of our new technique was to presoak the donor cornea in balanced salt solution (BSS) for 30?min before manual dissection. The cornea was placed on an artificial anterior chamber and pressure in the chamber was maintained at 80?mm?Hg. The limbus of the donor cornea was incised to the same depth as the central corneal thickness. Lamellar dissection was started with the short side of the Morlet dissector (Duckworth & Kent Ltd) and completed using the lamellar (less sharp) end of the Morlet dissector. Outcomes of 10 consecutive cases of thin manually dissected DSEK (TMDSEK) were prospectively analysed for thickness and visual outcome.ResultsMean graft thicknesses measured less than 100??m at 1 month post surgery (mean thickness 90.7??m, range 48-137??m, SD 29.96??m). Presoaked donor corneal pachymetry was strongly negatively correlated with graft thickness (correlation r=-0.75, P<0.05).DiscussionOur dissection technique achieves consistently thin endothelial donor corneal grafts that can be safely produced with minimal financial investment and no limitations on surgical time. This technique is likely to be of significant importance for a large proportion of the eye centres where microkeratomes may not be routinely available

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More information

e-pub ahead of print date: 14 March 2014
Published date: 14 March 2014
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 363336
URI: https://eprints.soton.ac.uk/id/eprint/363336
ISSN: 0950-222X
PURE UUID: 76a9f2c5-9be1-4187-97dd-012b913ddb58
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395

Catalogue record

Date deposited: 24 Mar 2014 14:39
Last modified: 20 Jul 2019 00:55

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Contributors

Author: M. Tsatsos
Author: Aris Konstantopoulos
Author: Parwez Hossain ORCID iD
Author: D. Anderson

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