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Monodisperse Water-in-Oil-in-Water (W/O/W) double emulsion droplets as uniform compartments for high-throughput analysis via flow cytometry

Monodisperse Water-in-Oil-in-Water (W/O/W) double emulsion droplets as uniform compartments for high-throughput analysis via flow cytometry
Monodisperse Water-in-Oil-in-Water (W/O/W) double emulsion droplets as uniform compartments for high-throughput analysis via flow cytometry
Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed
402-413
Yan, Jing
b4d1b3dd-452b-4eec-9f64-ecae39b55a62
Bauer, Wolfgang-Andreas
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Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Hollfelder, Florian
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Kaminski, Clemens
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Huck, Wilhelm
4d73f1f9-23ca-48fc-8ac2-992d66bd5546
Yan, Jing
b4d1b3dd-452b-4eec-9f64-ecae39b55a62
Bauer, Wolfgang-Andreas
8777bfb7-eb99-4505-9b75-8200faae534e
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Hollfelder, Florian
a9f01280-f05d-4057-b5d7-85eac249e477
Kaminski, Clemens
48d4a7b5-3cd5-4b7e-ba84-fe6b323945d2
Huck, Wilhelm
4d73f1f9-23ca-48fc-8ac2-992d66bd5546

Yan, Jing, Bauer, Wolfgang-Andreas, Fischlechner, Martin, Hollfelder, Florian, Kaminski, Clemens and Huck, Wilhelm (2013) Monodisperse Water-in-Oil-in-Water (W/O/W) double emulsion droplets as uniform compartments for high-throughput analysis via flow cytometry. Micromachines, 4 (4), 402-413. (doi:10.3390/mi4040402).

Record type: Article

Abstract

Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed

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Published date: 3 December 2013

Identifiers

Local EPrints ID: 363356
URI: http://eprints.soton.ac.uk/id/eprint/363356
PURE UUID: 0ddd7d0d-44b1-4f26-825d-401349c109bc

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Date deposited: 24 Mar 2014 14:20
Last modified: 14 Mar 2024 16:23

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Contributors

Author: Jing Yan
Author: Wolfgang-Andreas Bauer
Author: Martin Fischlechner
Author: Florian Hollfelder
Author: Clemens Kaminski
Author: Wilhelm Huck

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