The University of Southampton
University of Southampton Institutional Repository

DNase I footprinting

DNase I footprinting
DNase I footprinting
Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction
978-1-60327-417-3
153-172
Springer
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Cardew, Antonia S. and Fox, Keith R. (2009) DNase I footprinting. In, Drug-DNA Interaction Protocols. (Methods in Molecular Biology, , (doi:10.1007/978-1-60327-418-0_10), 613) New York, US. Springer, pp. 153-172. (doi:10.1007/978-1-60327-418-0_10).

Record type: Book Section

Abstract

Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction

Full text not available from this repository.

More information

Published date: 2009
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 363656
URI: https://eprints.soton.ac.uk/id/eprint/363656
ISBN: 978-1-60327-417-3
PURE UUID: 5158ae16-96d5-4029-a4a2-49eb693f668c
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 28 Mar 2014 16:24
Last modified: 06 Jun 2018 13:16

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×