DNase I footprinting
DNase I footprinting
Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction
978-1-60327-417-3
153-172
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
2009
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Cardew, Antonia S. and Fox, Keith R.
(2009)
DNase I footprinting.
In,
Drug-DNA Interaction Protocols.
(Methods in Molecular Biology, 613)
New York, US.
Springer, .
(doi:10.1007/978-1-60327-418-0_10).
Record type:
Book Section
Abstract
Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction
This record has no associated files available for download.
More information
Published date: 2009
Organisations:
Molecular and Cellular
Identifiers
Local EPrints ID: 363656
URI: http://eprints.soton.ac.uk/id/eprint/363656
ISBN: 978-1-60327-417-3
PURE UUID: 5158ae16-96d5-4029-a4a2-49eb693f668c
Catalogue record
Date deposited: 28 Mar 2014 16:24
Last modified: 08 Jan 2022 02:33
Export record
Altmetrics
Contributors
Author:
Antonia S. Cardew
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
