Scaleable micro-cavity bilayer lipid membrane array for parallel ion channel recording
Scaleable micro-cavity bilayer lipid membrane array for parallel ion channel recording
A compact, scalable and high-throughput bilayer ion channel recording platform capable of simultaneous data acquisition from multiple bilayers is presented. Microfluidic chips house micro-cavities over which bilayers are made; each connected to a custom-made compact electronic readout circuit based on ASICs (Application-Specific Integrated Circuits). The micro-cavities are fabricated using a simple dry-film resist process on a glass wafer. Single 15 mm × 15 mm glass chips contain four separately addressable bilayers, each with integrated Ag/AgCl electrodes. The number of bilayers is scaled by increasing the number of ASICs and four-cavity chips. Each chip can be cleaned and re-used many times and the cavity-suspended lipid bilayers are stable for up to 10 days. System performance is demonstrated with simultaneous electrical recordings of the ion channels gramicidin A and alpha-hemolysin in multiple bilayers.
bilayer lipid membrane (BLM), ion channel, electrophysiology, parallel platform, micro-cavity, dry film resists
76-82
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Thei, Federico
c5014b97-007e-482d-87ad-1d43162ac2eb
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
August 2014
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Thei, Federico
c5014b97-007e-482d-87ad-1d43162ac2eb
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Saha, Shimul C., Thei, Federico, de Planque, Maurits R.R. and Morgan, Hywel
(2014)
Scaleable micro-cavity bilayer lipid membrane array for parallel ion channel recording.
Sensors and Actuators B: Chemical, 199, .
(doi:10.1016/j.snb.2014.03.111).
Abstract
A compact, scalable and high-throughput bilayer ion channel recording platform capable of simultaneous data acquisition from multiple bilayers is presented. Microfluidic chips house micro-cavities over which bilayers are made; each connected to a custom-made compact electronic readout circuit based on ASICs (Application-Specific Integrated Circuits). The micro-cavities are fabricated using a simple dry-film resist process on a glass wafer. Single 15 mm × 15 mm glass chips contain four separately addressable bilayers, each with integrated Ag/AgCl electrodes. The number of bilayers is scaled by increasing the number of ASICs and four-cavity chips. Each chip can be cleaned and re-used many times and the cavity-suspended lipid bilayers are stable for up to 10 days. System performance is demonstrated with simultaneous electrical recordings of the ion channels gramicidin A and alpha-hemolysin in multiple bilayers.
Text
Saha_SensorsActuatorsB_2014.pdf
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More information
e-pub ahead of print date: 8 April 2014
Published date: August 2014
Keywords:
bilayer lipid membrane (BLM), ion channel, electrophysiology, parallel platform, micro-cavity, dry film resists
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 363681
URI: http://eprints.soton.ac.uk/id/eprint/363681
ISSN: 0925-4005
PURE UUID: 37074aff-998e-465d-9107-1e1bfb67cd08
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Date deposited: 30 Mar 2014 11:02
Last modified: 15 Mar 2024 03:18
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Contributors
Author:
Shimul C. Saha
Author:
Federico Thei
Author:
Maurits R.R. de Planque
Author:
Hywel Morgan
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