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Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications

Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications
Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications
We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C scaffolds are fabricated by selectively increasing the material's surface water affinity through lithography and oxygen plasma treatment, providing free bonds for attachment of hydrophilic biomolecules. The micro-engineered constructs were tested as culture scaffolds for rat ventricular fibroblasts and neonatal myocytes (NRVM), toward modeling the unique anisotropic architecture of native cardiac tissue. The scaffolds induced the patterning of extracellular matrix compounds and therefore of the cells, which demonstrated substantial alignment compared to typical unstructured cultures. Ca2+ cycling properties of the NRVM measured at rates of stimulation 0.5–2 Hz were significantly modified with a shorter time to peak and time to 90% decay, and a larger fluorescence amplitude (p < 0.001). The proposed technique is compatible with standard cell culturing protocols and exhibits long-term pattern durability. Moreover, it allows the integration of monitoring modalities into the micro-engineered substrates for a comprehensive interrogation of physiological parameters
1758-5082
25004
Trantidou, T.
1a2705ad-366d-432b-be5f-523d1303930c
Rao, C.
20f2d12e-20a6-4c86-9174-f1e7a7441ad3
Barrett, H.
53d7e7c3-ad3d-4409-bf4f-5794ac8ef872
Camelliti, P.
a66d563b-ed94-474c-8290-50202c3dc833
Pinto, K.
b71ebda1-8fbb-43f2-b925-1e27b8de8259
Yacoub, M.H.
34f26ea5-d1b6-47d6-b750-16ed7aaac019
Athanasiou, T.
545580db-4c19-49fd-8bf0-819b0fce81ed
Toumazou, C.
52728165-8fe5-4c54-9fad-e9ccc4423dd6
Terracciano, C.M.
79bb2862-f4c4-42b1-929c-3043b5bc8794
Prodromakis, T.
d58c9c10-9d25-4d22-b155-06c8437acfbf
Trantidou, T.
1a2705ad-366d-432b-be5f-523d1303930c
Rao, C.
20f2d12e-20a6-4c86-9174-f1e7a7441ad3
Barrett, H.
53d7e7c3-ad3d-4409-bf4f-5794ac8ef872
Camelliti, P.
a66d563b-ed94-474c-8290-50202c3dc833
Pinto, K.
b71ebda1-8fbb-43f2-b925-1e27b8de8259
Yacoub, M.H.
34f26ea5-d1b6-47d6-b750-16ed7aaac019
Athanasiou, T.
545580db-4c19-49fd-8bf0-819b0fce81ed
Toumazou, C.
52728165-8fe5-4c54-9fad-e9ccc4423dd6
Terracciano, C.M.
79bb2862-f4c4-42b1-929c-3043b5bc8794
Prodromakis, T.
d58c9c10-9d25-4d22-b155-06c8437acfbf

Trantidou, T., Rao, C., Barrett, H., Camelliti, P., Pinto, K., Yacoub, M.H., Athanasiou, T., Toumazou, C., Terracciano, C.M. and Prodromakis, T. (2014) Selective hydrophilic modification of Parylene C films: a new approach to cell micro-patterning for synthetic biology applications Biofabrication, 6, (2), p. 25004. (doi:10.1088/1758-5082/6/2/025004). (PMID:24658120).

Record type: Article

Abstract

We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C scaffolds are fabricated by selectively increasing the material's surface water affinity through lithography and oxygen plasma treatment, providing free bonds for attachment of hydrophilic biomolecules. The micro-engineered constructs were tested as culture scaffolds for rat ventricular fibroblasts and neonatal myocytes (NRVM), toward modeling the unique anisotropic architecture of native cardiac tissue. The scaffolds induced the patterning of extracellular matrix compounds and therefore of the cells, which demonstrated substantial alignment compared to typical unstructured cultures. Ca2+ cycling properties of the NRVM measured at rates of stimulation 0.5–2 Hz were significantly modified with a shorter time to peak and time to 90% decay, and a larger fluorescence amplitude (p < 0.001). The proposed technique is compatible with standard cell culturing protocols and exhibits long-term pattern durability. Moreover, it allows the integration of monitoring modalities into the micro-engineered substrates for a comprehensive interrogation of physiological parameters

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Published date: March 2014
Organisations: Nanoelectronics and Nanotechnology

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Local EPrints ID: 364120
URI: http://eprints.soton.ac.uk/id/eprint/364120
ISSN: 1758-5082
PURE UUID: ab2f8376-2db8-4f8d-8817-92610c0c6957

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Date deposited: 07 Apr 2014 07:44
Last modified: 18 Jul 2017 02:35

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Contributors

Author: T. Trantidou
Author: C. Rao
Author: H. Barrett
Author: P. Camelliti
Author: K. Pinto
Author: M.H. Yacoub
Author: T. Athanasiou
Author: C. Toumazou
Author: C.M. Terracciano
Author: T. Prodromakis

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