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A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation
A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation
Scientific research and progress, particularly in the drug discovery and regenerative medicine fields, is typically dependent on suitable animal models to develop new and improved clinical therapies for injuries and diseases. In vivo model systems are frequently utilised, but these models are expensive, highly complex and pose a number of ethical considerations leading to the development and use of a number of alternative ex vivo model systems. The ex vivo embryonic chick long bone and limb bud models have been utilised in the scientific research field as a model to understand skeletal development for over eighty years. The rapid development of avian skeletal tissues, coupled with the ease of experimental manipulation, availability of genome sequence and the presence of multiple cell and tissue types has seen such model systems gain significant research interest in the last few years in the tissue engineering field. The models have been explored both as systems for understanding the developmental bone niche and as potential testing tools for tissue engineering strategies for bone repair and regeneration. This review details the evolution of the chick limb organ culture system and presents recent innovative developments and emerging techniques and technologies applied to these models that are aiding our understanding of skeletal developmental and regenerative medicine research and application.
chick limb organotypic culture, ex vivo model, embryonic femur, micro-computed tomography, osteogenesis, chondrogenesis, chorioallantoic membrane assay, bone development and regeneration
91-106
Smith, Emma L.
a5cfde95-6ee5-41df-a21c-a3df3fcd92ec
Kanczler, Janos M.
7e06be24-613a-42dd-9d78-1079ecd5553d
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Smith, Emma L.
a5cfde95-6ee5-41df-a21c-a3df3fcd92ec
Kanczler, Janos M.
7e06be24-613a-42dd-9d78-1079ecd5553d
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Smith, Emma L., Kanczler, Janos M. and Oreffo, Richard O.C. (2013) A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation. European Cells & Materials, 26, 91-106. (PMID:24027022)

Record type: Article

Abstract

Scientific research and progress, particularly in the drug discovery and regenerative medicine fields, is typically dependent on suitable animal models to develop new and improved clinical therapies for injuries and diseases. In vivo model systems are frequently utilised, but these models are expensive, highly complex and pose a number of ethical considerations leading to the development and use of a number of alternative ex vivo model systems. The ex vivo embryonic chick long bone and limb bud models have been utilised in the scientific research field as a model to understand skeletal development for over eighty years. The rapid development of avian skeletal tissues, coupled with the ease of experimental manipulation, availability of genome sequence and the presence of multiple cell and tissue types has seen such model systems gain significant research interest in the last few years in the tissue engineering field. The models have been explored both as systems for understanding the developmental bone niche and as potential testing tools for tissue engineering strategies for bone repair and regeneration. This review details the evolution of the chick limb organ culture system and presents recent innovative developments and emerging techniques and technologies applied to these models that are aiding our understanding of skeletal developmental and regenerative medicine research and application.

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Published date: 11 September 2013
Keywords: chick limb organotypic culture, ex vivo model, embryonic femur, micro-computed tomography, osteogenesis, chondrogenesis, chorioallantoic membrane assay, bone development and regeneration
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 364235
URI: http://eprints.soton.ac.uk/id/eprint/364235
PURE UUID: 6a6a49cb-bcc7-422c-832b-0373a618a0d2
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 10 Apr 2014 11:57
Last modified: 28 Oct 2023 01:46

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Contributors

Author: Emma L. Smith
Author: Janos M. Kanczler

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