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Reduced ability to recover from spindle disruption and loss of kinetochore spindle assembly checkpoint proteins in oocytes from aged mice.

Reduced ability to recover from spindle disruption and loss of kinetochore spindle assembly checkpoint proteins in oocytes from aged mice.
Reduced ability to recover from spindle disruption and loss of kinetochore spindle assembly checkpoint proteins in oocytes from aged mice.
Currently, maternal aging in women, based on mouse models, is thought to raise oocyte aneuploidy rates, because chromosome cohesion deteriorates during prophase arrest, and Sgo2, a protector of centromeric cohesion, is lost. Here we show that the most common mouse strain, C57Bl6/J, is resistant to maternal aging, showing little increase in aneuploidy or Sgo2 loss. Instead it demonstrates significant kinetochore-associated loss in the spindle assembly checkpoint protein Mad2 and phosphorylated Aurora C, which is involved in microtubule-kinetochore error correction. Their loss affects the fidelity of bivalent segregation but only when spindle organization is impaired during oocyte maturation. These findings have an impact clinically regarding the handling of human oocytes ex vivo during assisted reproductive techniques and suggest there is a genetic basis to aneuploidy susceptibility.
1538-4101
1-10
Yun, Y
aaa5ab77-a61c-4dcb-910b-76996272c6c8
Holt, JE
c2b8b241-75af-4c44-972b-af9508fcfc2a
Lane, SIR
8e80111f-5012-4950-a228-dfb8fb9df52d
McLaughlin, EA
fcbf5f45-aa3b-4883-a84e-fc28781874b6
Merriman, JA
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, KT
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Yun, Y
aaa5ab77-a61c-4dcb-910b-76996272c6c8
Holt, JE
c2b8b241-75af-4c44-972b-af9508fcfc2a
Lane, SIR
8e80111f-5012-4950-a228-dfb8fb9df52d
McLaughlin, EA
fcbf5f45-aa3b-4883-a84e-fc28781874b6
Merriman, JA
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, KT
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4

Yun, Y, Holt, JE, Lane, SIR, McLaughlin, EA, Merriman, JA and Jones, KT (2014) Reduced ability to recover from spindle disruption and loss of kinetochore spindle assembly checkpoint proteins in oocytes from aged mice. Cell Cycle, 13 (12), 1-10. (PMID:24758999)

Record type: Article

Abstract

Currently, maternal aging in women, based on mouse models, is thought to raise oocyte aneuploidy rates, because chromosome cohesion deteriorates during prophase arrest, and Sgo2, a protector of centromeric cohesion, is lost. Here we show that the most common mouse strain, C57Bl6/J, is resistant to maternal aging, showing little increase in aneuploidy or Sgo2 loss. Instead it demonstrates significant kinetochore-associated loss in the spindle assembly checkpoint protein Mad2 and phosphorylated Aurora C, which is involved in microtubule-kinetochore error correction. Their loss affects the fidelity of bivalent segregation but only when spindle organization is impaired during oocyte maturation. These findings have an impact clinically regarding the handling of human oocytes ex vivo during assisted reproductive techniques and suggest there is a genetic basis to aneuploidy susceptibility.

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More information

Published date: 23 April 2014
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 364423
URI: http://eprints.soton.ac.uk/id/eprint/364423
ISSN: 1538-4101
PURE UUID: 9313340e-f3b1-4626-a590-4181bbe3b8de
ORCID for SIR Lane: ORCID iD orcid.org/0000-0002-8155-0981
ORCID for KT Jones: ORCID iD orcid.org/0000-0002-0294-0851

Catalogue record

Date deposited: 29 Apr 2014 09:55
Last modified: 29 Oct 2019 01:39

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Contributors

Author: Y Yun
Author: JE Holt
Author: SIR Lane ORCID iD
Author: EA McLaughlin
Author: JA Merriman
Author: KT Jones ORCID iD

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