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Compared with daily, weekly n-3 PUFA intake affects the incorporation of eicosapentaenoic acid and docosahexaenoic acid into platelets and mononuclear cells in humans

Compared with daily, weekly n-3 PUFA intake affects the incorporation of eicosapentaenoic acid and docosahexaenoic acid into platelets and mononuclear cells in humans
Compared with daily, weekly n-3 PUFA intake affects the incorporation of eicosapentaenoic acid and docosahexaenoic acid into platelets and mononuclear cells in humans
Consumption of oily fish is sporadic, whereas controlled intervention studies of n–3 (?-3) fatty acids usually provide capsules containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) as a daily dose. This methodologic study explored whether there are differences in the short-, medium-, and long-term incorporation of EPA and DHA into blood plasma and cells with the provision of identical amounts of EPA and DHA, equivalent to 2 oily fish servings per week (or 6.54 g/wk EPA and DHA), either intermittently (i.e., 1 portion twice per week) or continuously (i.e., divided into daily amounts). The study was part of a randomized, double-blind controlled intervention lasting 12 mo, with participants stratified by age and sex. There were 5 intervention groups, 2 of which are reported here: the 2 intermittent portions (2I) and 2 continuous portions (2C) groups. EPA and DHA were measured in plasma phosphatidylcholine, platelets, and blood mononuclear cells (MNCs) at 9 time points. Sixty-five participants completed the study (2I group, n = 30, mean age of 49.2 y; 2C group, n = 35, mean age of 50.6 y). The incorporation pattern over the 12-mo intervention was different between the 2 groups in all samples (P < 0.0001, time × treatment interaction). At the end of the 12-mo intervention, the 2C group had higher EPA, DHA, and EPA + DHA in platelets (all P < 0.01) and higher EPA and EPA + DHA in MNCs (both P < 0.05) compared with the 2I group. No significant differences were shown for plasma phosphatidylcholine EPA (P = 0.1), DHA (P = 0.15), EPA + DHA (P = 0.07), or MNC DHA (P = 0.06). In conclusion, the pattern of consumption does affect the incorporation of EPA and DHA into cells used as biomarkers of intake. The differences identified here need to be considered in the design of studies and when extrapolating results from continuous capsule-based intervention studies to dietary guidelines for oily fish consumption. This trial was registered at www.controlled-trials.com as ISRCTN48398526
0022-3166
667-672
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Walker, C.G.
612e7ef4-6bf9-42f6-9948-0ad00021fd49
Mander, A.P.
1368b569-3778-4088-b2ce-6857a9740331
West, A.L.
1f80ca4e-cf66-4e7c-976e-0f6a200df213
Gambell, J.
78b40b90-92c0-48d0-b2f3-198e95e6ab33
Madden, J.
48adfcd0-5977-4ebb-96bd-4e80b56fcd94
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Walker, C.G.
612e7ef4-6bf9-42f6-9948-0ad00021fd49
Mander, A.P.
1368b569-3778-4088-b2ce-6857a9740331
West, A.L.
1f80ca4e-cf66-4e7c-976e-0f6a200df213
Gambell, J.
78b40b90-92c0-48d0-b2f3-198e95e6ab33
Madden, J.
48adfcd0-5977-4ebb-96bd-4e80b56fcd94
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2

Browning, L.M., Walker, C.G., Mander, A.P., West, A.L., Gambell, J., Madden, J., Calder, P.C. and Jebb, S.A. (2014) Compared with daily, weekly n-3 PUFA intake affects the incorporation of eicosapentaenoic acid and docosahexaenoic acid into platelets and mononuclear cells in humans. Journal of Nutrition, 144 (5), 667-672. (doi:10.3945/?jn.113.186346). (PMID:24647395)

Record type: Article

Abstract

Consumption of oily fish is sporadic, whereas controlled intervention studies of n–3 (?-3) fatty acids usually provide capsules containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) as a daily dose. This methodologic study explored whether there are differences in the short-, medium-, and long-term incorporation of EPA and DHA into blood plasma and cells with the provision of identical amounts of EPA and DHA, equivalent to 2 oily fish servings per week (or 6.54 g/wk EPA and DHA), either intermittently (i.e., 1 portion twice per week) or continuously (i.e., divided into daily amounts). The study was part of a randomized, double-blind controlled intervention lasting 12 mo, with participants stratified by age and sex. There were 5 intervention groups, 2 of which are reported here: the 2 intermittent portions (2I) and 2 continuous portions (2C) groups. EPA and DHA were measured in plasma phosphatidylcholine, platelets, and blood mononuclear cells (MNCs) at 9 time points. Sixty-five participants completed the study (2I group, n = 30, mean age of 49.2 y; 2C group, n = 35, mean age of 50.6 y). The incorporation pattern over the 12-mo intervention was different between the 2 groups in all samples (P < 0.0001, time × treatment interaction). At the end of the 12-mo intervention, the 2C group had higher EPA, DHA, and EPA + DHA in platelets (all P < 0.01) and higher EPA and EPA + DHA in MNCs (both P < 0.05) compared with the 2I group. No significant differences were shown for plasma phosphatidylcholine EPA (P = 0.1), DHA (P = 0.15), EPA + DHA (P = 0.07), or MNC DHA (P = 0.06). In conclusion, the pattern of consumption does affect the incorporation of EPA and DHA into cells used as biomarkers of intake. The differences identified here need to be considered in the design of studies and when extrapolating results from continuous capsule-based intervention studies to dietary guidelines for oily fish consumption. This trial was registered at www.controlled-trials.com as ISRCTN48398526

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Published date: May 2014
Organisations: Human Development & Health

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Local EPrints ID: 364487
URI: http://eprints.soton.ac.uk/id/eprint/364487
ISSN: 0022-3166
PURE UUID: 3b82bf51-18f9-4473-ac11-24f369fe46cb
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 01 May 2014 13:41
Last modified: 15 Mar 2024 02:50

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Contributors

Author: L.M. Browning
Author: C.G. Walker
Author: A.P. Mander
Author: A.L. West
Author: J. Gambell
Author: J. Madden
Author: P.C. Calder ORCID iD
Author: S.A. Jebb

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