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Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring

Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring
Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring
Purpose

Prenatal undernutrition followed by postweaning feeding of a high-fat diet results in obesity in the adult offspring. In this study, we investigated whether diet-induced thermogenesis is altered as a result of such nutritional mismatch.

Methods

Female MF-1 mice were fed a normal protein (NP, 18 % casein) or a protein-restricted (PR, 9 % casein) diet throughout pregnancy and lactation. After weaning, male offspring of both groups were fed either a high-fat diet (HF; 45 % kcal fat) or standard chow (C, 7 % kcal fat) to generate the NP/C, NP/HF, PR/C and PR/HF adult offspring groups (n = 7–11 per group).

Results

PR/C and NP/C offspring have similar body weights at 30 weeks of age. Postweaning HF feeding resulted in significantly heavier NP/HF offspring (P < 0.01), but not in PR/HF offspring, compared with their chow-fed counterparts. However, the PR/HF offspring exhibited greater adiposity (P < 0.01) v the NP/HF group. The NP/HF offspring had increased energy expenditure and increased mRNA expression of uncoupling protein-1 and ?-3 adrenergic receptor in the interscapular brown adipose tissue (iBAT) compared with the NP/C mice (both at P < 0.01). No such differences in energy expenditure and iBAT gene expression were observed between the PR/HF and PR/C offspring.

Conclusions

These data suggest that a mismatch between maternal diet during pregnancy and lactation, and the postweaning diet of the offspring, can attenuate diet-induced thermogenesis in the iBAT, resulting in the development of obesity in adulthood.
1436-6207
1-9
Sellayah, Dyan
4b637ae6-a2f1-4eb6-8bd6-5155c58ac424
Dib, Lea
855f749e-285d-4bf7-a28b-f1c2b08238b3
Anthony, Frederick W.
28a46159-500c-48fe-8c55-ef57e034cbeb
Watkins, Adam J.
2d535c61-2df0-4410-a1b4-3aa1be5a43bb
Fleming, Tom P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Sellayah, Dyan
4b637ae6-a2f1-4eb6-8bd6-5155c58ac424
Dib, Lea
855f749e-285d-4bf7-a28b-f1c2b08238b3
Anthony, Frederick W.
28a46159-500c-48fe-8c55-ef57e034cbeb
Watkins, Adam J.
2d535c61-2df0-4410-a1b4-3aa1be5a43bb
Fleming, Tom P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e

Sellayah, Dyan, Dib, Lea, Anthony, Frederick W., Watkins, Adam J., Fleming, Tom P., Hanson, Mark A. and Cagampang, Felino R. (2014) Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring. European Journal of Nutrition, 1-9. (doi:10.1007/s00394-014-0657-4). (PMID:24481689)

Record type: Article

Abstract

Purpose

Prenatal undernutrition followed by postweaning feeding of a high-fat diet results in obesity in the adult offspring. In this study, we investigated whether diet-induced thermogenesis is altered as a result of such nutritional mismatch.

Methods

Female MF-1 mice were fed a normal protein (NP, 18 % casein) or a protein-restricted (PR, 9 % casein) diet throughout pregnancy and lactation. After weaning, male offspring of both groups were fed either a high-fat diet (HF; 45 % kcal fat) or standard chow (C, 7 % kcal fat) to generate the NP/C, NP/HF, PR/C and PR/HF adult offspring groups (n = 7–11 per group).

Results

PR/C and NP/C offspring have similar body weights at 30 weeks of age. Postweaning HF feeding resulted in significantly heavier NP/HF offspring (P < 0.01), but not in PR/HF offspring, compared with their chow-fed counterparts. However, the PR/HF offspring exhibited greater adiposity (P < 0.01) v the NP/HF group. The NP/HF offspring had increased energy expenditure and increased mRNA expression of uncoupling protein-1 and ?-3 adrenergic receptor in the interscapular brown adipose tissue (iBAT) compared with the NP/C mice (both at P < 0.01). No such differences in energy expenditure and iBAT gene expression were observed between the PR/HF and PR/C offspring.

Conclusions

These data suggest that a mismatch between maternal diet during pregnancy and lactation, and the postweaning diet of the offspring, can attenuate diet-induced thermogenesis in the iBAT, resulting in the development of obesity in adulthood.

Full text not available from this repository.

More information

e-pub ahead of print date: 31 January 2014
Organisations: Human Development & Health, Centre for Biological Sciences

Identifiers

Local EPrints ID: 364777
URI: http://eprints.soton.ac.uk/id/eprint/364777
ISSN: 1436-6207
PURE UUID: c8207ff6-2ffe-4355-bc7f-13d619734114
ORCID for Mark A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Felino R. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853

Catalogue record

Date deposited: 08 May 2014 14:33
Last modified: 20 Jul 2019 01:07

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