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Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer

Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer
Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer
We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.
1932-1058
1-9
Katis, Ioannis
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Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Faust, Saul N.
f97df780-9f9b-418e-b349-7adf63e150c1
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Voegeli, David
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Faust, Saul N.
f97df780-9f9b-418e-b349-7adf63e150c1
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Voegeli, David
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

Katis, Ioannis, Holloway, Judith A., Madsen, Jens, Faust, Saul N., Garbis, Spiros D., Smith, Peter J.S., Voegeli, David, Bader, Dan L., Eason, Robert W. and Sones, Collin L. (2014) Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer. Biomicrofluidics, 8 (3), 1-9. (doi:10.1063/1.4878696). (PMID:24926392)

Record type: Article

Abstract

We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.

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Accepted/In Press date: 8 May 2014
e-pub ahead of print date: 19 May 2014
Published date: May 2014
Organisations: Optoelectronics Research Centre, Centre for Biological Sciences

Identifiers

Local EPrints ID: 364881
URI: https://eprints.soton.ac.uk/id/eprint/364881
ISSN: 1932-1058
PURE UUID: 8ba4e418-6945-4bca-940b-9cc7a110867d
ORCID for Ioannis Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Judith A. Holloway: ORCID iD orcid.org/0000-0002-2268-3071
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645
ORCID for Saul N. Faust: ORCID iD orcid.org/0000-0003-3410-7642
ORCID for Spiros D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853
ORCID for David Voegeli: ORCID iD orcid.org/0000-0003-3457-7177
ORCID for Dan L. Bader: ORCID iD orcid.org/0000-0002-1208-3507
ORCID for Robert W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

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Date deposited: 15 May 2014 12:19
Last modified: 07 Jun 2019 00:32

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