The University of Southampton
University of Southampton Institutional Repository

Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer

Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer
Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer
We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care (POC) diagnostic sensors. Paper was chosen as the ideal receiver due to its inherent biocompatibility, wicking properties, wide availability and price, all of which make it an efficient and suitable platform for POC diagnostic sensors. A modified LIFT procedure, referred to as Dynamic Release Layer (DRL-LIFT), has been employed to ensure the viability of the biomolecules post-transfer. The laser used was a KrF excimer operating at 248 nm with a repetition rate of 1 Hz, pulse duration of ~10 ns, and delivering a maximum energy of ~400 mJ per pulse. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed. The functionality and immunological reactivity of the LIFT-printed antibodies was confirmed by developing and demonstrating an Enzyme Linked Immunosorbent Assay and establishing the standard calibration curve for the LIFT-printed pixels. Additionally, it was shown that the localisation of the LIFT-printed pixels and immobilisation of the antibodies, a pre-requisite for paper-based diagnostic devices, was maintained throughout the wet-bench process which further justifies the spatial patterning ability of LIFT. This work demonstrates that LIFT is a technique capable of transferring antibodies onto a paper substrate accurately, reproducibly and with minimal loss of biochemical viability.
Katis, I.N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Holloway, J.A.
cb83a730-2d2f-4d46-822e-1e68e96a937b
Madsen, J.
59643478-6c66-40ab-8536-1b1bbe4a51ea
Faust, S.N.
0789760a-3367-4529-bd32-94bbd5b1d71c
Garbis, S.D.
70752871-dee6-4cea-be1d-e9b97fe9af5d
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Voegeli, D.
bdd87594-4203-469a-9ef7-603a8c2882ae
Bader, D.L.
e7086f10-f647-47b5-b2a5-42fef92cc049
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, C.L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
Katis, I.N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Holloway, J.A.
cb83a730-2d2f-4d46-822e-1e68e96a937b
Madsen, J.
59643478-6c66-40ab-8536-1b1bbe4a51ea
Faust, S.N.
0789760a-3367-4529-bd32-94bbd5b1d71c
Garbis, S.D.
70752871-dee6-4cea-be1d-e9b97fe9af5d
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Voegeli, D.
bdd87594-4203-469a-9ef7-603a8c2882ae
Bader, D.L.
e7086f10-f647-47b5-b2a5-42fef92cc049
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, C.L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

Katis, I.N., Holloway, J.A., Madsen, J., Faust, S.N., Garbis, S.D., Smith, P.J.S., Voegeli, D., Bader, D.L., Eason, R.W. and Sones, C.L. (2014) Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer. Materials Research Society Spring Meeting E-MRS '14. 26 - 30 May 2014.

Record type: Conference or Workshop Item (Paper)

Abstract

We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care (POC) diagnostic sensors. Paper was chosen as the ideal receiver due to its inherent biocompatibility, wicking properties, wide availability and price, all of which make it an efficient and suitable platform for POC diagnostic sensors. A modified LIFT procedure, referred to as Dynamic Release Layer (DRL-LIFT), has been employed to ensure the viability of the biomolecules post-transfer. The laser used was a KrF excimer operating at 248 nm with a repetition rate of 1 Hz, pulse duration of ~10 ns, and delivering a maximum energy of ~400 mJ per pulse. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed. The functionality and immunological reactivity of the LIFT-printed antibodies was confirmed by developing and demonstrating an Enzyme Linked Immunosorbent Assay and establishing the standard calibration curve for the LIFT-printed pixels. Additionally, it was shown that the localisation of the LIFT-printed pixels and immobilisation of the antibodies, a pre-requisite for paper-based diagnostic devices, was maintained throughout the wet-bench process which further justifies the spatial patterning ability of LIFT. This work demonstrates that LIFT is a technique capable of transferring antibodies onto a paper substrate accurately, reproducibly and with minimal loss of biochemical viability.

Full text not available from this repository.

More information

e-pub ahead of print date: 2014
Venue - Dates: Materials Research Society Spring Meeting E-MRS '14, 2014-05-26 - 2014-05-30
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 365103
URI: http://eprints.soton.ac.uk/id/eprint/365103
PURE UUID: b784b47e-b5f4-4c52-8a2b-7900ec433f06
ORCID for I.N. Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for P.J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853
ORCID for R.W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 22 May 2014 10:59
Last modified: 18 Feb 2021 17:23

Export record

Contributors

Author: I.N. Katis ORCID iD
Author: J.A. Holloway
Author: J. Madsen
Author: S.N. Faust
Author: S.D. Garbis
Author: P.J.S. Smith ORCID iD
Author: D. Voegeli
Author: D.L. Bader
Author: R.W. Eason ORCID iD
Author: C.L. Sones

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×