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Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups

Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups
Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups
Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (?7.5?pmol?cm?2).
cyclic voltammetry, electrochemistry, proteins, solid-phase synthesis
0947-6539
5550-5554
Wright, Emma
e9c8593a-52e3-4fdc-a028-350d2c4c810c
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Bloodworth, Sally
943160fc-1b70-4c29-b2e3-b7785cee8a0c
Kilburn, Jeremy D.
e64ded70-825a-40ec-816b-c4605e007e7a
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Wright, Emma
e9c8593a-52e3-4fdc-a028-350d2c4c810c
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Bloodworth, Sally
943160fc-1b70-4c29-b2e3-b7785cee8a0c
Kilburn, Jeremy D.
e64ded70-825a-40ec-816b-c4605e007e7a
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Wright, Emma, Sosna, Maciej, Bloodworth, Sally, Kilburn, Jeremy D. and Bartlett, Philip N. (2014) Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine groups. Chemistry - A European Journal, 20 (19), 5550-5554. (doi:10.1002/chem.201400246).

Record type: Article

Abstract

Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (?7.5?pmol?cm?2).

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e-pub ahead of print date: 10 April 2014
Published date: 5 May 2014
Keywords: cyclic voltammetry, electrochemistry, proteins, solid-phase synthesis
Organisations: Chemistry

Identifiers

Local EPrints ID: 365133
URI: http://eprints.soton.ac.uk/id/eprint/365133
ISSN: 0947-6539
PURE UUID: 23b38291-f9f0-49e8-b546-5c0e60296ff8
ORCID for Sally Bloodworth: ORCID iD orcid.org/0000-0003-2219-3635
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 23 May 2014 16:02
Last modified: 03 Dec 2019 02:04

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Contributors

Author: Emma Wright
Author: Maciej Sosna
Author: Jeremy D. Kilburn

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