The University of Southampton
University of Southampton Institutional Repository

Fish oil supplementation decreases oxidative stress but does not affect platelet-activating factor bioactivity in lungs of asthmatic rats

Fish oil supplementation decreases oxidative stress but does not affect platelet-activating factor bioactivity in lungs of asthmatic rats
Fish oil supplementation decreases oxidative stress but does not affect platelet-activating factor bioactivity in lungs of asthmatic rats
Dietary fish oil supplementation increases the content of n-3 polyunsaturated fatty acids (PUFA) in cellular membranes. The highly unsaturated nature of n-3 PUFA could result in an enhanced lipid peroxidation in the oxidative environment characteristic of asthma. The oxidative reaction cascade culminates in an increased production of components associated to oxidative stress and of an important proinflammatory mediator platelet-activating factor (PAF)-like lipid. We evaluated the effect of fish oil supplementation in asthmatic rats upon the PAF bioactivity and parameters related to oxidative stress in the lung. Fish oil supplementation of asthmatic rats resulted in lower concentrations of nitrite (1.719 ± 0.137 vs. 2.454 ± 0.163 nmol/mL) and lipid hydroperoxide (72.190 ± 7.327 vs. 120.200 ± 11.270 nmol/mg protein). In asthmatic animals, fish oil increased the activities of superoxide dismutase (EC 1.15.1.1) (33.910 ± 2.325 vs. 24.110 ± 0.618 U/mg protein) and glutathione peroxidase (EC 1.11.1.9) (164.100 ± 31.250 vs. 12.590 ± 5.234 U/mg protein). However, fish oil did not affect PAF bioactivity in lung tissue of asthmatic rats (0.545 ± 0.098 340/380 vs. 0.669 ± 0.101 340/380 nm ratio). Considering the two-step process—oxidative stress and PAF bioactivity—fish oil exhibited a divergent action on these aspects of asthmatic inflammation, since the supplement lowered oxidative stress in the lungs of asthmatic rats, presenting an antioxidant effect, but did not affect PAF bioactivity. This suggests a dual effect of fish oil on oxidative stress and inflammation in asthma.
0024-4201
665-675
Zanatta, A.L.
753e71ea-dbba-4b1d-8416-8ebac202f5fa
Miranda, D.T.
686682d2-2d5a-44eb-b092-88475952c439
Dias, B.C.
0140a82f-fc9e-4eb7-b217-a14c1d17a384
Campos, R.M.
e8c3cf3a-d50e-43e7-861e-bf4d92ae24fc
Massaro, M.C.
83fcd591-2077-4c87-82b6-bf7505f299c3
Michelotto, P.V. Jr
32369552-5d62-4400-b1b1-08bdab7ee4ab
West, Annette L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A.
a5d17b06-7790-44f3-80b4-1e33cc4446e9
Zanatta, A.L.
753e71ea-dbba-4b1d-8416-8ebac202f5fa
Miranda, D.T.
686682d2-2d5a-44eb-b092-88475952c439
Dias, B.C.
0140a82f-fc9e-4eb7-b217-a14c1d17a384
Campos, R.M.
e8c3cf3a-d50e-43e7-861e-bf4d92ae24fc
Massaro, M.C.
83fcd591-2077-4c87-82b6-bf7505f299c3
Michelotto, P.V. Jr
32369552-5d62-4400-b1b1-08bdab7ee4ab
West, Annette L.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A.
a5d17b06-7790-44f3-80b4-1e33cc4446e9

Zanatta, A.L., Miranda, D.T., Dias, B.C., Campos, R.M., Massaro, M.C., Michelotto, P.V. Jr, West, Annette L., Miles, Elizabeth A., Calder, P.C. and Nishiyama, A. (2014) Fish oil supplementation decreases oxidative stress but does not affect platelet-activating factor bioactivity in lungs of asthmatic rats. Lipids, 49 (7), 665-675. (doi:10.1007/s11745-014-3914-7). (PMID:24858941)

Record type: Article

Abstract

Dietary fish oil supplementation increases the content of n-3 polyunsaturated fatty acids (PUFA) in cellular membranes. The highly unsaturated nature of n-3 PUFA could result in an enhanced lipid peroxidation in the oxidative environment characteristic of asthma. The oxidative reaction cascade culminates in an increased production of components associated to oxidative stress and of an important proinflammatory mediator platelet-activating factor (PAF)-like lipid. We evaluated the effect of fish oil supplementation in asthmatic rats upon the PAF bioactivity and parameters related to oxidative stress in the lung. Fish oil supplementation of asthmatic rats resulted in lower concentrations of nitrite (1.719 ± 0.137 vs. 2.454 ± 0.163 nmol/mL) and lipid hydroperoxide (72.190 ± 7.327 vs. 120.200 ± 11.270 nmol/mg protein). In asthmatic animals, fish oil increased the activities of superoxide dismutase (EC 1.15.1.1) (33.910 ± 2.325 vs. 24.110 ± 0.618 U/mg protein) and glutathione peroxidase (EC 1.11.1.9) (164.100 ± 31.250 vs. 12.590 ± 5.234 U/mg protein). However, fish oil did not affect PAF bioactivity in lung tissue of asthmatic rats (0.545 ± 0.098 340/380 vs. 0.669 ± 0.101 340/380 nm ratio). Considering the two-step process—oxidative stress and PAF bioactivity—fish oil exhibited a divergent action on these aspects of asthmatic inflammation, since the supplement lowered oxidative stress in the lungs of asthmatic rats, presenting an antioxidant effect, but did not affect PAF bioactivity. This suggests a dual effect of fish oil on oxidative stress and inflammation in asthma.

This record has no associated files available for download.

More information

e-pub ahead of print date: 25 May 2014
Published date: July 2014
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 366395
URI: http://eprints.soton.ac.uk/id/eprint/366395
ISSN: 0024-4201
PURE UUID: 01560e25-216b-4e3e-a128-15221a740f46
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 27 Jun 2014 14:32
Last modified: 15 Mar 2024 02:50

Export record

Altmetrics

Contributors

Author: A.L. Zanatta
Author: D.T. Miranda
Author: B.C. Dias
Author: R.M. Campos
Author: M.C. Massaro
Author: P.V. Jr Michelotto
Author: Annette L. West
Author: P.C. Calder ORCID iD
Author: A. Nishiyama

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×