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Direct detection of DNA on gold structured planar substrates by Raman microscopy

Direct detection of DNA on gold structured planar substrates by Raman microscopy
Direct detection of DNA on gold structured planar substrates by Raman microscopy
Detection of DNA sequences is pivotal to many modern molecular diagnostic methods, but the ability to directly detect DNA sequences, without the need for signal amplification (such as by applying a polymerase chain reactions) is highly desirable. Here we investigate the potential for gold inverted pyramidal structures (also known as Klarite®) for DNA detection, as these simple chips containing the structured surface on a face could offer an improved format for DNA based diagnostic methods. Our strategy included optimization of the fabrication protocols to achieve flat gold surfaces within the inverted pyramidal surface and then a subsequent assessment of these substrates for direct DNA detection by Raman microscopy. These studies demonstrate for the first time the potential of these gold structured planar substrates for DNA analysis applications.
978-8-8872-3718-4
1-4
Carpignano, F.
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Grant-Jacob, J.A.
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Lamb, J.
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Pechstedt, K.
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Brocklesby, W.S.
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Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Carpignano, F.
e794e71c-1adf-407d-aa80-5b302bc39864
Grant-Jacob, J.A.
c5d144d8-3c43-4195-8e80-edd96bfda91b
Lamb, J.
b992c5c4-8291-47f6-83f5-e76f61b695b4
Pechstedt, K.
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Brocklesby, W.S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Carpignano, F., Grant-Jacob, J.A., Lamb, J., Pechstedt, K., Brocklesby, W.S. and Melvin, T. (2014) Direct detection of DNA on gold structured planar substrates by Raman microscopy. 2014 Fotonica AEIT Italian Conference on Photonics Technologies, Naples, Italy. 12 - 14 May 2014. pp. 1-4 . (doi:10.1109/Fotonica.2014.6843930).

Record type: Conference or Workshop Item (Paper)

Abstract

Detection of DNA sequences is pivotal to many modern molecular diagnostic methods, but the ability to directly detect DNA sequences, without the need for signal amplification (such as by applying a polymerase chain reactions) is highly desirable. Here we investigate the potential for gold inverted pyramidal structures (also known as Klarite®) for DNA detection, as these simple chips containing the structured surface on a face could offer an improved format for DNA based diagnostic methods. Our strategy included optimization of the fabrication protocols to achieve flat gold surfaces within the inverted pyramidal surface and then a subsequent assessment of these substrates for direct DNA detection by Raman microscopy. These studies demonstrate for the first time the potential of these gold structured planar substrates for DNA analysis applications.

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Published date: May 2014
Venue - Dates: 2014 Fotonica AEIT Italian Conference on Photonics Technologies, Naples, Italy, 2014-05-12 - 2014-05-14
Organisations: Optoelectronics Research Centre, Electronics & Computer Science

Identifiers

Local EPrints ID: 366971
URI: https://eprints.soton.ac.uk/id/eprint/366971
ISBN: 978-8-8872-3718-4
PURE UUID: 1b92b5fd-a8ce-4b58-99d5-c80cc48fa36a
ORCID for J.A. Grant-Jacob: ORCID iD orcid.org/0000-0002-4270-4247
ORCID for W.S. Brocklesby: ORCID iD orcid.org/0000-0002-2123-6712

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Date deposited: 16 Jul 2014 14:24
Last modified: 26 Nov 2019 02:05

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Contributors

Author: F. Carpignano
Author: J. Lamb
Author: K. Pechstedt
Author: W.S. Brocklesby ORCID iD
Author: T. Melvin

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