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Isolation and enrichment of Stro-1 immunoselected mesenchymal stem cells from adult human bone marrow

Isolation and enrichment of Stro-1 immunoselected mesenchymal stem cells from adult human bone marrow
Isolation and enrichment of Stro-1 immunoselected mesenchymal stem cells from adult human bone marrow
The availability of mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs) is vital to many of the tissue engineering strategies currently being developed for repairing bone and cartilage. One difficulty with using this cell population is that SSCs represent only a small fraction of the cells available from an individual patient's bone marrow sample, typically less than 1 in 10,000. Therefore, methods have been devised to enrich the proportion of MSCs obtained from a bone marrow sample using hybridoma cell lines to generate antibodies to cell surface antigens specific for MSCs. Stro-1 is the most widely targeted of these cell surface antigens. The protocol described overleaf is used to isolate and enrich the Stro-1 positive fraction of cells from a bone marrow aspirate to provide a sample enriched for MSCs for use in both in vitro and in vivo studies.
67-73
Williams, Emma L.
fbcdc734-fa2a-4741-9dd9-dab9413c6111
White, Kate
ad592ed1-c133-4e3a-abed-3803a6d5531c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Williams, Emma L.
fbcdc734-fa2a-4741-9dd9-dab9413c6111
White, Kate
ad592ed1-c133-4e3a-abed-3803a6d5531c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Williams, Emma L., White, Kate and Oreffo, Richard O.C. (2013) Isolation and enrichment of Stro-1 immunoselected mesenchymal stem cells from adult human bone marrow. Methods in Molecular Biology, 1035, 67-73. (doi:10.1007/978-1-62703-508-8_7). (PMID:23959983)

Record type: Article

Abstract

The availability of mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs) is vital to many of the tissue engineering strategies currently being developed for repairing bone and cartilage. One difficulty with using this cell population is that SSCs represent only a small fraction of the cells available from an individual patient's bone marrow sample, typically less than 1 in 10,000. Therefore, methods have been devised to enrich the proportion of MSCs obtained from a bone marrow sample using hybridoma cell lines to generate antibodies to cell surface antigens specific for MSCs. Stro-1 is the most widely targeted of these cell surface antigens. The protocol described overleaf is used to isolate and enrich the Stro-1 positive fraction of cells from a bone marrow aspirate to provide a sample enriched for MSCs for use in both in vitro and in vivo studies.

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Published date: 13 June 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 367175
URI: http://eprints.soton.ac.uk/id/eprint/367175
PURE UUID: 73ef7dec-d0a4-486e-934c-b390a522e155
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 18 Aug 2014 11:28
Last modified: 09 Jan 2022 03:02

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Contributors

Author: Emma L. Williams
Author: Kate White

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