The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

The scale-up of a tissue engineered porous hydroxyapatite polymer composite scaffold for use in bone repair: an ovine femoral condyle defect study

The scale-up of a tissue engineered porous hydroxyapatite polymer composite scaffold for use in bone repair: an ovine femoral condyle defect study
The scale-up of a tissue engineered porous hydroxyapatite polymer composite scaffold for use in bone repair: an ovine femoral condyle defect study
The development of an osteogenic bone graft substitute has important practical and cost implications in many branches of medicine where bone regeneration is required. Previous in vitro and small animal (murine) in vivo studies highlighted a porous hydroxyapatite/poly (dl‐lactic acid) composite scaffold in combination with skeletal stem cells (SSCs) as a potential bone graft substitute candidate. The aim of the current study was to scale up the bone cell‐scaffold construct to large animals and examine the potential for repair of a critical‐sized defect via an ovine model. SSC seeded scaffolds (and unseeded scaffold controls) were implanted bilaterally into ovine femoral condyle critical defects for 3 months. A parallel in vitro analysis of ovine SSC seeded scaffolds was also performed. Post mortem mechanical indentation testing showed the bone strengths of the defect sites were 20% (controls) and 11% (SSC seeded scaffolds) those of normal cancellous bone (p < 0.01). MicroCT analysis demonstrated new bone formation within all defects with a mean increase of 13.4% in the control scaffolds over the SSC seeded scaffolds (p = 0.14). Histological examination confirmed these findings, with enhanced quality new bone within the control defects. This study highlights important issues and steps to overcome in scale‐up and translation of tissue engineered products. The scaffold demonstrated encouraging results as an osteoconductive matrix; however, further work is required with cellular protocols before any human trials.
1549-3296
1346-1356
Tayton, Edward R.
2c0e26d2-1f5f-498d-9ff7-d22200f19b3a
Purcell, Matthew
69a3121a-489d-4b8d-9249-9829727d1a06
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Howdle, Steven M.
ec70f53e-a5df-4e99-9da2-f90582dde80b
Shakesheff, Kevin M.
5b9ed879-e2c5-4c62-bb38-abd2bb4960ac
Goodship, Allen
b727629e-3d3d-4c30-a6b8-ff4e08d3f775
Blunn, Gordon
e443f1ef-65ff-4628-be6b-cfb2f232421f
Fowler, Darren
7b2b3b09-9582-44ae-ae90-db236a9dae37
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Tayton, Edward R.
2c0e26d2-1f5f-498d-9ff7-d22200f19b3a
Purcell, Matthew
69a3121a-489d-4b8d-9249-9829727d1a06
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Howdle, Steven M.
ec70f53e-a5df-4e99-9da2-f90582dde80b
Shakesheff, Kevin M.
5b9ed879-e2c5-4c62-bb38-abd2bb4960ac
Goodship, Allen
b727629e-3d3d-4c30-a6b8-ff4e08d3f775
Blunn, Gordon
e443f1ef-65ff-4628-be6b-cfb2f232421f
Fowler, Darren
7b2b3b09-9582-44ae-ae90-db236a9dae37
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Tayton, Edward R., Purcell, Matthew, Smith, James O., Lanham, Stuart, Howdle, Steven M., Shakesheff, Kevin M., Goodship, Allen, Blunn, Gordon, Fowler, Darren, Dunlop, Douglas G. and Oreffo, Richard O.C. (2014) The scale-up of a tissue engineered porous hydroxyapatite polymer composite scaffold for use in bone repair: an ovine femoral condyle defect study. Journal of Biomedical Materials Research Part A, 103 (4), 1346-1356. (doi:10.1002/jbm.a.35279).

Record type: Article

Abstract

The development of an osteogenic bone graft substitute has important practical and cost implications in many branches of medicine where bone regeneration is required. Previous in vitro and small animal (murine) in vivo studies highlighted a porous hydroxyapatite/poly (dl‐lactic acid) composite scaffold in combination with skeletal stem cells (SSCs) as a potential bone graft substitute candidate. The aim of the current study was to scale up the bone cell‐scaffold construct to large animals and examine the potential for repair of a critical‐sized defect via an ovine model. SSC seeded scaffolds (and unseeded scaffold controls) were implanted bilaterally into ovine femoral condyle critical defects for 3 months. A parallel in vitro analysis of ovine SSC seeded scaffolds was also performed. Post mortem mechanical indentation testing showed the bone strengths of the defect sites were 20% (controls) and 11% (SSC seeded scaffolds) those of normal cancellous bone (p < 0.01). MicroCT analysis demonstrated new bone formation within all defects with a mean increase of 13.4% in the control scaffolds over the SSC seeded scaffolds (p = 0.14). Histological examination confirmed these findings, with enhanced quality new bone within the control defects. This study highlights important issues and steps to overcome in scale‐up and translation of tissue engineered products. The scaffold demonstrated encouraging results as an osteoconductive matrix; however, further work is required with cellular protocols before any human trials.

Text
The scale-up of a tissue engineered porous hydroxyapatite polymer composite scaffold for use in bone repair An ovine femoral condyle defect study.pdf - Other
Restricted to Repository staff only
Request a copy

More information

e-pub ahead of print date: 16 July 2014
Published date: 22 July 2014
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 367260
URI: http://eprints.soton.ac.uk/id/eprint/367260
ISSN: 1549-3296
PURE UUID: c3a10206-ca23-421d-af47-1357571c51a6
ORCID for Stuart Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 29 Jul 2014 14:33
Last modified: 21 Nov 2021 02:49

Export record

Altmetrics

Contributors

Author: Edward R. Tayton
Author: Matthew Purcell
Author: James O. Smith
Author: Stuart Lanham ORCID iD
Author: Steven M. Howdle
Author: Kevin M. Shakesheff
Author: Allen Goodship
Author: Gordon Blunn
Author: Darren Fowler
Author: Douglas G. Dunlop

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×